| BrdU-Hoechst flow cytometry reveals regulation of human lymphocyte growth by donor-age-related growth fraction and transition rate. | |
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MedLine Citation:
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PMID: 4055906 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An improved BrdU-Hoechst flow assay was applied to cell kinetic studies of human lymphocyte cultures during a 24-96 hr interval after PHA stimulation. The assay shows that the duration of the initial lag phase and the proportions of noncycling cells increase as a function of donor age, whereas the rates of transition from each cell cycle compartment to the next decrease. Cell cycle arrest occurs in the first S and G2 phase after stimulation of lymphocytes from a 75-year-old donor but not from younger donors. The data are consistent with several models of cell cycle kinetics, so long as these models are modified to include a fraction of noncycling cells in each cell cycle compartment. |
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Authors:
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M Kubbies; D Schindler; H Hoehn; P S Rabinovitch |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of cellular physiology Volume: 125 ISSN: 0021-9541 ISO Abbreviation: J. Cell. Physiol. Publication Date: 1985 Nov |
Date Detail:
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Created Date: 1985-11-22 Completed Date: 1985-11-22 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 229-34 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aging* Blood Donors* Bromodeoxyuridine / diagnostic use* Cell Cycle / drug effects Cell Division Dose-Response Relationship, Drug Flow Cytometry / methods* Humans Lymphocyte Activation Lymphocytes / cytology* Models, Biological Phytohemagglutinins / diagnostic use |
| Grant Support | |
ID/Acronym/Agency:
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AG-01751/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Phytohemagglutinins; 59-14-3/Bromodeoxyuridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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