Document Detail


BrdU-Hoechst flow cytometry reveals regulation of human lymphocyte growth by donor-age-related growth fraction and transition rate.
MedLine Citation:
PMID:  4055906     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An improved BrdU-Hoechst flow assay was applied to cell kinetic studies of human lymphocyte cultures during a 24-96 hr interval after PHA stimulation. The assay shows that the duration of the initial lag phase and the proportions of noncycling cells increase as a function of donor age, whereas the rates of transition from each cell cycle compartment to the next decrease. Cell cycle arrest occurs in the first S and G2 phase after stimulation of lymphocytes from a 75-year-old donor but not from younger donors. The data are consistent with several models of cell cycle kinetics, so long as these models are modified to include a fraction of noncycling cells in each cell cycle compartment.
Authors:
M Kubbies; D Schindler; H Hoehn; P S Rabinovitch
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  125     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1985 Nov 
Date Detail:
Created Date:  1985-11-22     Completed Date:  1985-11-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  229-34     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aging*
Blood Donors*
Bromodeoxyuridine / diagnostic use*
Cell Cycle / drug effects
Cell Division
Dose-Response Relationship, Drug
Flow Cytometry / methods*
Humans
Lymphocyte Activation
Lymphocytes / cytology*
Models, Biological
Phytohemagglutinins / diagnostic use
Grant Support
ID/Acronym/Agency:
AG-01751/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/Phytohemagglutinins; 59-14-3/Bromodeoxyuridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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