Document Detail

BrdU birth dating can produce errors in cell fate specification in chick brain development.
MedLine Citation:
PMID:  22859704     Owner:  NLM     Status:  MEDLINE    
Birth dating neurons with bromodeoxyuridine (BrdU) labeling is an established method widely employed by neurobiologists to study cell proliferation in embryonic, postnatal, and adult brain. Birth dating studies in the chick dorsal telencephalon and the mammalian striatum have suggested that these structures develop in a strikingly similar manner, in which neurons with the same birth date aggregate to form "isochronic clusters." Here we show that isochronic cluster formation in the chick dorsal telencephalon is an artifact. In embryos given standardly employed doses of BrdU, we observed isochronic clusters but found that clusters were absent with BrdU doses close to the limits of detection. In addition, in situ hybridization experiments established that neurons in the clusters display errors in cell type specification: BrdU cell clusters in nidopallium adopted a mesopallial neuronal fate, mesopallial clusters were misspecified as nidopallial cells, and in some instances, the BrdU clusters failed to express neuronal differentiation markers characteristic of the dorsal telencephalon. These results demonstrate that the chick dorsal telencephalon does not develop by isochronic cluster formation and highlight the need to test the integrity of BrdU-treated tissue with gene expression markers of regional and cell type identity.
Joanna J Rowell; Clifton W Ragsdale
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-08-02
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  60     ISSN:  1551-5044     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-29     Completed Date:  2013-01-07     Revised Date:  2013-11-06    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  801-10     Citation Subset:  IM    
Department of Neurobiology, University of Chicago, Chicago, IL 60637, USA.
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MeSH Terms
Bromodeoxyuridine / adverse effects,  analysis*
Chick Embryo / cytology*,  drug effects,  metabolism,  ultrastructure*
Gene Expression Regulation, Developmental / drug effects
Staining and Labeling / methods*
Telencephalon / cytology*,  drug effects,  metabolism,  ultrastructure*
Reg. No./Substance:

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