Document Detail


Brazilian marine sponge Polymastia janeirensis induces apoptotic cell death in human U138MG glioma cell line, but not in a normal cell culture.
MedLine Citation:
PMID:  18454276     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Marine sponges have been prominently featured in the area of cancer research. Here, we examined the anti-proliferative effects of crude extracts (aqueous and organic) of the Brazilian marine sponge Polymastia janeirensis in the U138MG human glioma cell line. Moreover, we examined the effects of extracts on selective cytotoxicity in the glioma cells in comparison with a normal cell culture. Exposure of glioma cells to treatments (24 h) resulted in cell number decrease at all doses tested, with both aqueous and organic extracts (IC(50) <20 and <30 microg/ml, respectively). Parallel to this result, sponge extracts reduced glioma cell viability (IC(50) <15 microg/ml for both extracts). However, higher doses (50 and 100 microg/ml) induced a stronger cytotoxic effect when compared to the lower dose tested (10 microg/ml), inhibiting more than 80% of cellular growth and viability. Propidium iodide uptake and flow cytometry analysis further showed that sponge extracts caused necrosis in the glioma cell line at higher doses, while a high percentage of apoptotic glioma cells were observed at 10 microg/ml. Moreover, apoptosis was prevented by the pan-caspase inhibitor Z-VAD, suggesting that marine sponge extracts, at lower doses, induce caspase-dependent apoptosis in U138MG glioma cells. Surprisingly the extracts herein tested were more effective than temozolomide, a potent inductor of apoptosis used for the treatment of malignant gliomas. Furthermore, our results suggested a selectivity cytotoxic effect on glioma cell line in comparison with a normal cell culture, since the effect on viability found in glioma cells was not observed in astrocyte cultures with the lower dose (10 microg/ml). Thus, this marine sponge may be considered a good candidate for development of new cancer medicines with antitumor activity against gliomas.
Authors:
Mario Luiz Conte da Frota; Elizandra Braganhol; Andrés Delgado Canedo; Fabio Klamt; Miriam Anders Apel; Beatriz Mothes; Cléa Lerner; Ana Maria Oliveira Battastini; Amélia Teresinha Henriques; José Cláudio Fonseca Moreira
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-05-03
Journal Detail:
Title:  Investigational new drugs     Volume:  27     ISSN:  0167-6997     ISO Abbreviation:  Invest New Drugs     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2008-12-23     Completed Date:  2009-03-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8309330     Medline TA:  Invest New Drugs     Country:  United States    
Other Details:
Languages:  eng     Pagination:  13-20     Citation Subset:  IM    
Affiliation:
Centro de Estudos em Estresse Oxidativo (CEEO), ICBS, UFRGS, Porto Alegre, Brazil. mfrotajr@yahoo.com.br
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Chloromethyl Ketones / antagonists & inhibitors
Animals
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects
Astrocytes / drug effects
Brazil
Cell Line, Transformed
Cell Line, Tumor
Complex Mixtures / pharmacology*
Dacarbazine / analogs & derivatives,  pharmacology
Glioma / drug therapy*
Humans
Porifera / chemistry*
Chemical
Reg. No./Substance:
0/Amino Acid Chloromethyl Ketones; 0/Antineoplastic Agents; 0/Complex Mixtures; 0/benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone; 4342-03-4/Dacarbazine; 85622-93-1/temozolomide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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