| Brains of apolipoprotein E deficient mice fed vitamin E deficient diets show alteration in handling alpha tocopherol injected into the cerebral ventricles. | |
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MedLine Citation:
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PMID: 19446300 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Abnormal function of apolipoprotein E (apoE) has been implicated in the incidence of some neurological disorders including dementia. Our recent experiments have shown that apoE deficiency alters the dynamics of alpha tocopherol (vitamin E) handling by brain. In the current investigation, we examined the uptake and retention of tritium-labeled alpha tocopherol that was injected into the lateral cerebral ventricles of apoE-deficient and wild type mice that were fed vitamin E-deficient diet. Eighteen weeks-old, male mice were fed vitamin E-deficient diets for 28 weeks. Labeled cholesterol was injected with the radioactive tocopherol and the cholesterol counts were used as internal standard. After an equilibration time of 48 h, radioactive alpha tocopherol levels in most brain regions were higher in apoE deficient animals when compared with the wild type. Along with our other data, this suggests that the clearance of vitamin E is slower in apoE-deficient brains. Nearly all of the injected alpha tocopherol was unchanged in the brains of both apoE-deficient and wild type animals (even with the additional dietary stress of vitamin E deficiency) suggesting low turnover rate of tocopherol in brain. The data strongly suggest that apoE is a key protein involved with the transport and/or retention of alpha tocopherol in brain. |
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Authors:
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Govind T Vatassery; W Ed Smith; Hung T Quach |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Date: 2009-05-14 |
Journal Detail:
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Title: Journal of the neurological sciences Volume: 283 ISSN: 1878-5883 ISO Abbreviation: J. Neurol. Sci. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-07-20 Completed Date: 2009-10-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375403 Medline TA: J Neurol Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 195-8 Citation Subset: IM |
Affiliation:
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Research Service and the GRECC Program, VA Medical Center, Minneapolis, Minnesota 55417, USA. vatas001@umn.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apolipoproteins E / deficiency*, genetics Brain / drug effects*, metabolism* Carbon Radioisotopes Cholesterol / metabolism Chromatography, Liquid Diet Liver / drug effects, metabolism Male Mice Mice, Inbred C57BL Mice, Knockout Tritium Vitamin E Deficiency / metabolism* Vitamins / pharmacokinetics* alpha-Tocopherol / pharmacokinetics* |
| Chemical | |
Reg. No./Substance:
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0/Apolipoproteins E; 0/Carbon Radioisotopes; 0/Vitamins; 10028-17-8/Tritium; 57-88-5/Cholesterol; 59-02-9/alpha-Tocopherol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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