Document Detail

Brain-type creatine kinase BB-CK interacts with the Golgi Matrix Protein GM130 in early prophase.
MedLine Citation:
PMID:  17036164     Owner:  NLM     Status:  MEDLINE    
Creatine kinase (CK) isoenzymes are essential for storing, buffering and intracellular transport of "energy-rich" phosphate compounds in tissues with fluctuating high energy demand such as muscle, brain and other tissues and cells where CK is expressed. In brain and many non-muscle cells, ubiquitous cytosolic "brain-type" BB-CK and ubiquitous mitochondrial CK (uMtCK) act as components of a phosphocreatine shuttle to maintain cellular energy pools and distribute energy flux. To date, still relatively little is known about direct coupling of functional dimeric BB-CK with other partner proteins or enzymes that are important for cell function. Using a global yeast two-hybrid (Y2H) screen with monomeric B-CK as bait and a representative brain cDNA library to search for interaction partners of B-CK with proteins of the brain, we repeatedly identified the cis-Golgi Matrix protein (GM130) as recurrent interacting partner of B-CK. Since HeLa cells also express both BB-CK and GM130, we subsequently used this cellular model system to verify and characterize the BB-CK-GM130 complex by GST-pulldown experiments, as well as by in vivo co-localization studies with confocal microscopy. Using dividing HeLa cells, we report here for the first time that GM130 and BB-CK co-localize specifically in a transient fashion during early prophase of mitosis, when GM130 plays an important role in Golgi fragmentation that starts also at early prophase. These data may shed new light on BB-CK function for energy provision for Golgi-fragmentation that is initiated by cell signalling cascades in the early phases of mitosis.
Tanja S Bürklen; Alain Hirschy; Theo Wallimann
Related Documents :
830654 - Nuclear matrix of hela s3 cells. polypeptide composition during adenovirus infection an...
1402804 - A monoclonal antibody recognizes a human cell surface glycoprotein involved in measles ...
18792104 - The ability to survive mitosis in the presence of microtubule poisons differs significa...
24373334 - Visualizing hepatitis b virus with biarsenical labelling in living cells.
2503624 - Comparison of human and monkey cells for the ability to attenuate transcripts that begi...
24736114 - Synthesis and biological evaluation of cinnamido linked benzophenone hybrids as tubulin...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-10-12
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  297     ISSN:  0300-8177     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-15     Completed Date:  2007-06-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  53-64     Citation Subset:  IM    
Institute of Cell Biology, HPM D24, ETH ZURICH, Schafmattstr. 18, Zurich 8093, Switzerland.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acids
Autoantigens / chemistry,  metabolism*
Brefeldin A / pharmacology
Creatine Kinase, BB Form / metabolism*
Golgi Apparatus / drug effects,  metabolism
Hela Cells
Liver / drug effects,  metabolism
Membrane Proteins / chemistry,  metabolism*
Prophase* / drug effects
Protein Binding / drug effects
Protein Structure, Tertiary
Protein Transport / drug effects
Recombinant Fusion Proteins / metabolism
Two-Hybrid System Techniques
Reg. No./Substance:
0/Amino Acids; 0/Autoantigens; 0/Golgin subfamily A member 2; 0/Membrane Proteins; 0/Recombinant Fusion Proteins; 20350-15-6/Brefeldin A; EC Kinase, BB Form

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  High expression of APOBEC3G in patients infected with hepatitis C virus.
Next Document:  Phytocomponent p-hydroxycinnamic acid stimulates bone formation and inhibits bone resorption in rat ...