| Brain-type creatine kinase BB-CK interacts with the Golgi Matrix Protein GM130 in early prophase. | |
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MedLine Citation:
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PMID: 17036164 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Creatine kinase (CK) isoenzymes are essential for storing, buffering and intracellular transport of "energy-rich" phosphate compounds in tissues with fluctuating high energy demand such as muscle, brain and other tissues and cells where CK is expressed. In brain and many non-muscle cells, ubiquitous cytosolic "brain-type" BB-CK and ubiquitous mitochondrial CK (uMtCK) act as components of a phosphocreatine shuttle to maintain cellular energy pools and distribute energy flux. To date, still relatively little is known about direct coupling of functional dimeric BB-CK with other partner proteins or enzymes that are important for cell function. Using a global yeast two-hybrid (Y2H) screen with monomeric B-CK as bait and a representative brain cDNA library to search for interaction partners of B-CK with proteins of the brain, we repeatedly identified the cis-Golgi Matrix protein (GM130) as recurrent interacting partner of B-CK. Since HeLa cells also express both BB-CK and GM130, we subsequently used this cellular model system to verify and characterize the BB-CK-GM130 complex by GST-pulldown experiments, as well as by in vivo co-localization studies with confocal microscopy. Using dividing HeLa cells, we report here for the first time that GM130 and BB-CK co-localize specifically in a transient fashion during early prophase of mitosis, when GM130 plays an important role in Golgi fragmentation that starts also at early prophase. These data may shed new light on BB-CK function for energy provision for Golgi-fragmentation that is initiated by cell signalling cascades in the early phases of mitosis. |
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Authors:
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Tanja S Bürklen; Alain Hirschy; Theo Wallimann |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-10-12 |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 297 ISSN: 0300-8177 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-03-15 Completed Date: 2007-06-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: Netherlands |
Other Details:
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Languages: eng Pagination: 53-64 Citation Subset: IM |
Affiliation:
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Institute of Cell Biology, HPM D24, ETH ZURICH, Schafmattstr. 18, Zurich 8093, Switzerland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids Animals Autoantigens / chemistry, metabolism* Brefeldin A / pharmacology Creatine Kinase, BB Form / metabolism* Golgi Apparatus / drug effects, metabolism Hela Cells Humans Liver / drug effects, metabolism Membrane Proteins / chemistry, metabolism* Prophase* / drug effects Protein Binding / drug effects Protein Structure, Tertiary Protein Transport / drug effects Rats Recombinant Fusion Proteins / metabolism Two-Hybrid System Techniques |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Autoantigens; 0/Golgin subfamily A member 2; 0/Membrane Proteins; 0/Recombinant Fusion Proteins; 20350-15-6/Brefeldin A; EC 2.7.3.2/Creatine Kinase, BB Form |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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