| Brain tumor stem cells from an adenoid glioblastoma multiforme. | |
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MedLine Citation:
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PMID: 19398857 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Brain tumors may arise from and contain cancer stem cells (CSCs) capable of self-renewal, proliferation, and differentiation that recapitulate the parent tumor. These CSCs are thought to be important in gliomagenesis. Detection of CSCs invading the adjacent brain regions is important for the diagnosis and effective treatment of glioblastoma multiforme (GBM). A 57-year-old man presented with an adenoid GBM, and underwent resection of the tumor. Multipotent, self-renewing cells derived from the human adenoid GBM were isolated and identified with the tumor-derived stem cell surface antigen CD133 from whole autopsied patient's brain. Tumorsphere culture and flow cytometric analysis revealed that 1.02-2.32% of the cells were positive for CD133. Transplantation of cultured tumorspheres into the mice brain resulted in the formation of well-defined tumor masses after 12 weeks. The histological and immunohistochemical characteristics of the xenograft were identical to those of the parent tumor. Examination of the patient's brain at autopsy showed CD133-positive cells were identified in the brain regions adjacent to the tumor, suggesting that CD133-positive CSCs might be localized to the vascular niche. Methods to localize CSCs may open new approaches for the treatment of brain tumors. |
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Authors:
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Naoki Oka; Akio Soeda; Shinji Noda; Toru Iwama |
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Publication Detail:
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Type: Case Reports; Journal Article |
Journal Detail:
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Title: Neurologia medico-chirurgica Volume: 49 ISSN: 1349-8029 ISO Abbreviation: Neurol. Med. Chir. (Tokyo) Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-04-28 Completed Date: 2009-07-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0400775 Medline TA: Neurol Med Chir (Tokyo) Country: Japan |
Other Details:
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Languages: eng Pagination: 146-50; discussion 150-1 Citation Subset: IM |
Affiliation:
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Department of Neurosurgery, Gifu University School of Medicine. noka@1kw.jp |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD / analysis, metabolism Brain Neoplasms / metabolism*, pathology Cell Culture Techniques Cell Proliferation Cerebral Arteries / pathology Disease Models, Animal Glioblastoma / metabolism*, pathology Glycoproteins / analysis, metabolism Graft Survival / physiology Humans Male Mice Mice, SCID Middle Aged Neoplasm Invasiveness / pathology, physiopathology Neoplastic Stem Cells / metabolism*, transplantation* Peptides / analysis, metabolism Spheroids, Cellular Stem Cell Transplantation / methods* Transplantation, Heterologous / methods* Tumor Cells, Cultured Tumor Markers, Biological / analysis, metabolism |
| Chemical | |
Reg. No./Substance:
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0/AC133 antigen; 0/Antigens, CD; 0/Glycoproteins; 0/Peptides; 0/Tumor Markers, Biological |
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