Document Detail


Brain tissue preparations for chromosomal PRINS labeling.
MedLine Citation:
PMID:  16861758     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The preparation of a somatic cell tissue is a key point of any successful molecular biology and genetic analysis of chromosome complement and genome variations. Current molecular biology investigations require an increased amount of different tissues to study. The genetic studies of the brain became an intriguing part of oncology, neurogenetics, and psychiatric genetics. The brain tissue processing for molecular cytogenetic analysis has special peculiarities quite different from the protocols used in the field. Despite some pilot cytogenetic studies of chromosome complement in mammalian brain, the specified protocol of the brain sample preparation for molecular cytogenetic analysis has not been thoroughly described. Here, we propose the detailed brain tissue processing protocol that could be used for different molecular cytogenetic approaches to study chromosome complement in interphase nuclei.
Authors:
Ivan Y Iourov; Svetlana G Vorsanova; Franck Pellestor; Yuri B Yurov
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  334     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2006  
Date Detail:
Created Date:  2006-07-24     Completed Date:  2006-08-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  123-32     Citation Subset:  IM    
Affiliation:
Research Center of Mental Health, Russian Academy of Medical Sciences, Moscow, Russia.
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MeSH Terms
Descriptor/Qualifier:
Adult
Brain / metabolism*
Cell Nucleus / metabolism
Cells, Cultured
Chromosomes, Human / genetics*
Fetus
Formaldehyde
Humans
Interphase / genetics
Paraffin Embedding
Primed In Situ Labeling / methods*
Quality Control
Tissue Fixation
Chemical
Reg. No./Substance:
50-00-0/Formaldehyde

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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