Document Detail


Brain sodium channels and ouabainlike compounds mediate central aldosterone-induced hypertension.
MedLine Citation:
PMID:  12933342     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Central nervous system (CNS) effects of mineralocorticoids participate in the development of salt-sensitive hypertension. In the brain, mineralocorticoids activate amiloride-sensitive sodium channels, and we hypothesized that this would lead to increased release of ouabainlike compounds (OLC) and thereby sympathetic hyperactivity and hypertension. In conscious Wistar rats, intracerebroventricular infusion of aldosterone at 300 or 900 ng/h in artificial cerebrospinal fluid (aCSF) with 0.145 M Na+ for 2 h did not change baseline mean arterial pressure (MAP), renal sympathetic nerve activity (RSNA), or heart rate (HR). Intracerebroventricular infusion of aCSF containing 0.16 M Na+ (versus 0.145 M Na+ in regular aCSF) did not change MAP or RSNA, but significant increases in MAP, RSNA, and HR were observed after intracerebroventricular infusion of aldosterone at 300 ng/h for 2 h. Intracerebroventricular infusion of aCSF containing 0.3 M Na+ increased MAP, RSNA, and HR significantly more after intracerebroventricular infusion of aldosterone versus vehicle. After intracerebroventricular infusion of aldosterone, the MAP, RSNA, and HR responses to intracerebroventricular infusion of aCSF containing 0.16 M Na+ were blocked by blockade of brain OLC with intracerebroventricular infusion of Fab fragments or of brain sodium channels with intracerebroventricular benzamil. Chronic intracerebroventricular infusion of aldosterone at 25 ng/h in aCSF with 0.15 M Na+ for 2 wk increased MAP by 15-20 mmHg and increased hypothalamic OLC by 30% and pituitary OLC by 60%. Benzamil blocked all these responses to aldosterone. These findings indicate that in the brain, mineralocorticoids activate brain sodium channels, with small increases in CSF Na+ leading to increases in brain OLC, sympathetic outflow, and blood pressure.
Authors:
Hao Wang; Bing S Huang; Frans H H Leenen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-08-21
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  285     ISSN:  0363-6135     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-11-17     Completed Date:  2003-12-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H2516-23     Citation Subset:  IM    
Affiliation:
Hypertension Unit, University of Ottawa Heart Institute, Ottawa, Ontario, Canada K1Y 4W7.
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MeSH Terms
Descriptor/Qualifier:
Aldosterone / pharmacology*
Amiloride / analogs & derivatives*,  pharmacology
Animals
Blood Pressure / drug effects,  physiology
Brain Chemistry / physiology*
Cardenolides
Digoxin / metabolism*
Heart Rate / drug effects,  physiology
Hypertension / chemically induced,  physiopathology*
Injections, Intraventricular
Male
Rats
Rats, Wistar
Saponins / metabolism*
Sodium / cerebrospinal fluid
Sodium Channels / metabolism*
Sympathetic Nervous System / drug effects,  physiology
Chemical
Reg. No./Substance:
0/Cardenolides; 0/Saponins; 0/Sodium Channels; 0/digoxin-like factors; 20830-75-5/Digoxin; 2609-46-3/Amiloride; 2898-76-2/benzamil; 52-39-1/Aldosterone; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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