Document Detail


Brain region specific alterations in the protein and mRNA levels of protein kinase A subunits in the post-mortem brain of teenage suicide victims.
MedLine Citation:
PMID:  15920506     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Protein kinase A (PKA), a critical component of the adenylyl cyclase signaling system, phosphorylates crucial proteins and has been implicated in the pathophysiology of depression and suicide. The objective of the study was to examine if changes in PKA activity or in the protein and messenger RNA (mRNA) expression of any of its subunits are related to the pathophysiology of teenage suicide. We determined PKA activity and the protein and mRNA expression of different subunits of PKA in cytosol and membrane fractions obtained from the prefrontal cortex, (PFC) hippocampus, and nucleus accumbens (NA) of post-mortem brain from 17 teenage suicide victims and 17 nonpsychiatric control subjects. PKA activity was significantly decreased in the PFC but not the hippocampus of teenage suicide victims as compared with controls. However, the protein and mRNA expression of only two PKA subunits, that is, PKA RIalpha and PKA RIbeta, but not any other subunits were significantly decreased in both membrane and cytosol fractions of the PFC and protein expression of RIalpha and RIbeta in the NA of teenage suicide victims as compared to controls. A decrease in protein and mRNA expression of two specific PKA subunits may be associated with the pathogenesis of teenage suicide, and this decrease may be brain region specific, which may be related to the specific behavioral functions associated with these brain areas. Whether these changes in PKA subunits are related to suicidal behavior or are a result of suicide or are specific to suicide is not clear at this point.
Authors:
Ghanshyam N Pandey; Yogesh Dwivedi; Xinguo Ren; Hooriyah S Rizavi; Amal C Mondal; Pradeep K Shukla; Robert R Conley
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology     Volume:  30     ISSN:  0893-133X     ISO Abbreviation:  Neuropsychopharmacology     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-09-22     Completed Date:  2005-11-29     Revised Date:  2011-05-18    
Medline Journal Info:
Nlm Unique ID:  8904907     Medline TA:  Neuropsychopharmacology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1548-56     Citation Subset:  IM    
Affiliation:
Department of Psychiatry, The Psychiatric Institute, University of Illinois at Chicago, Chicago, IL 60612, USA. Gnpandey@psych.uic.edu
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Analysis of Variance
Blotting, Western / methods
Brain / drug effects,  enzymology*,  pathology
Cell Membrane / drug effects,  enzymology
Cyclic AMP / pharmacokinetics
Cyclic AMP-Dependent Protein Kinases / genetics,  metabolism*
Cytosol / drug effects,  enzymology
Female
Humans
Male
Postmortem Changes
Protein Subunits / genetics,  metabolism
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction / methods
Suicide*
Tritium / pharmacokinetics
Grant Support
ID/Acronym/Agency:
K01 MH 01836/MH/NIMH NIH HHS; R01 MH 068777/MH/NIMH NIH HHS; R01 MH 48153/MH/NIMH NIH HHS
Chemical
Reg. No./Substance:
0/Protein Subunits; 0/RNA, Messenger; 10028-17-8/Tritium; 60-92-4/Cyclic AMP; EC 2.7.11.11/Cyclic AMP-Dependent Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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