| Brain prostaglandin formation is increased by alpha-synuclein gene-ablation during global ischemia. | |
| | |
MedLine Citation:
|
PMID: 18226447 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We have previously demonstrated that alpha-synuclein (Snca) gene ablation reduces brain arachidonic acid (20:4n-6) turnover rate in phospholipids through modulation of endoplasmic reticulum-localized acyl-CoA synthetase activity. Although 20:4n-6 is a precursor for prostaglandin (PG), Snca effect on PG levels is unknown. In the present study, we examined the effect of Snca ablation on brain PG level at basal conditions and following 30s of global ischemia. Brain PG were extracted with methanol, purified on C(18) cartridges, and analyzed by LC-MS/MS. We demonstrate, for the first time, that Snca gene ablation did not affect brain PG mass under normal physiological conditions. However, total PG mass and masses of individual PG were elevated approximately 2-fold upon global ischemia in the absence of Snca. These data are consistent with our previously observed reduction in 20:4n-6 recycling through endoplasmic reticulum-localized acyl-CoA synthetase in the absence of Snca, which may result in the increased 20:4n-6 availability for PG production in the absence of Snca during global ischemia and suggest a role for Snca in brain inflammatory response. |
| | |
Authors:
|
Mikhail Y Golovko; Eric J Murphy |
Related Documents
:
|
16009437 - Change in plasma volume during peak exercise in patients with cyanotic congenital heart... 2969677 - The differential response in atrial natriuretic peptide release during exercise in pati... 16159987 - Comparison of endothelial vasodilator function, inflammatory markers, and n-terminal pr... 1677937 - Renal neurohormonal and vascular responses to dynamic exercise in humans. 16276137 - The impact of exercise on body composition and nutritional intake in patients with hear... 2106267 - Amino acid metabolism after intense exercise. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2007-12-23 |
Journal Detail:
|
Title: Neuroscience letters Volume: 432 ISSN: 0304-3940 ISO Abbreviation: Neurosci. Lett. Publication Date: 2008 Feb |
Date Detail:
|
Created Date: 2008-02-18 Completed Date: 2008-06-12 Revised Date: 2013-06-06 |
Medline Journal Info:
|
Nlm Unique ID: 7600130 Medline TA: Neurosci Lett Country: Ireland |
Other Details:
|
Languages: eng Pagination: 243-7 Citation Subset: IM |
Affiliation:
|
Department of Pharmacology, Physiology, and Therapeutics, School of Medicine and Health Sciences, University of North Dakota, 501 N. Columbia Road, Grand Forks, ND 58202-9037, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Analysis of Variance Animals Chromatography, Liquid / methods Gene Expression Regulation / physiology Ischemia / metabolism*, pathology, physiopathology Male Mice Mice, Knockout Prostaglandins / classification, metabolism* Tandem Mass Spectrometry / methods alpha-Synuclein / deficiency* |
| Grant Support | |
ID/Acronym/Agency:
|
P20 RR 17699/RR/NCRR NIH HHS; P20 RR017699-010004/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Prostaglandins; 0/alpha-Synuclein |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Polyferric sulphate: preparation, characterisation and application in coagulation experiments.
Next Document: Sympathetic overactivity and plasma leptin levels in Rett syndrome.