Document Detail

Brain hypoxia studied in mouse central nervous system cultures. I. Sequential cellular changes.
MedLine Citation:
PMID:  1238876     Owner:  NLM     Status:  MEDLINE    
Heavily myelinated cultures of newborn mouse cerebellum were exposed to hypoxia and studied by electron microscopy. The cultures were placed in an incubating medium deprived of oxygen (95 to 97 per cent deprivation) for 7 to 60 minutes and fixed immediately for electron microscopy. The amount of oxygen present in the medium and time course of hypoxia were constantly monitored and recorded by an oxygen probe of polarographic oxygen sensor type and a recorder. In contrast to previous in vivo studies, this in vitro model of hypoxic cell injury has provided accurate information concerning the relationship between degree of hypoxia and cell lesion. Hypoxia affected neurons which showed "swelling" and disorganization of cristae in mitochondria and reduced cytoplasmic matrix density due to the dispersion of polysomes. A small population of neurons with an increased cytoplasmic density and "swollen" mitochondria was also noted. Clearing and degeneration of presynaptic terminals and postsynaptic dendrites were observed. After a longer period of hypoxia most neurons showed an extensive degenerative change consisting of rarefaction of cytoplasm and loss of cytoplasmic organelles. In contrast to neuronal changes, no structural alteration was observed in astrocytes and oligodendrocytes.
S U Kim
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Laboratory investigation; a journal of technical methods and pathology     Volume:  33     ISSN:  0023-6837     ISO Abbreviation:  Lab. Invest.     Publication Date:  1975 Dec 
Date Detail:
Created Date:  1976-03-01     Completed Date:  1976-03-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376617     Medline TA:  Lab Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  658-69     Citation Subset:  IM    
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MeSH Terms
Animals, Newborn
Cerebellar Diseases / pathology*
Cerebellum / ultrastructure*
Endoplasmic Reticulum / ultrastructure
Hypoxia, Brain / pathology*
Microscopy, Electron
Mitochondria / ultrastructure
Mitochondrial Swelling
Organ Culture Techniques
Synapses / ultrastructure
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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