Document Detail


Brain endo-oligopeptidase B: inactivation of LH-RH by hydrolysis of the Pro9-Gly-NH2(10) peptide bond.
MedLine Citation:
PMID:  6763542     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. The site of hydrolysis of rabbit brain endo-oligopeptidase B acting on luteinizing hormone-releasing hormone (LH-RH) was determined by isolating the products by chromatography on Aminex A-5 resin developed with pyridine-acetic acid buffer. The products [des-Gly-NH2(10)]-LH-RH, glycinamide and unhydrolyzed LH-RH were identified and shown to be homogeneous by amino acid analysis and high-voltage paper electrophoresis at pH 2.1 and 3.5 and recovered in yields of 58, 65 and 23%, respectively. 2. A sensitive analytical method for the measurement of 4-40 nmoles of glycinamide with an automatic amino acid analyzer was described. Aminex A-5 resin (0.90 x 15 cm) was eluted with sodium citrate buffer, pH 3.25 (0.2 N Na+) at 32 degrees C and ninhydrin was used for detection. 3. The data show that endo-oligopeptidase B acts as a post-proline cleaving enzyme that inactivates LH-RH by hydrolysis of the Pro9-Gly-NH2(10) peptide bond. The enzyme may participate in the metabolism of LH-RH in the central nervous system.
Authors:
A C Camargo; A C Spadaro; A R Martins; L J Greene
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.]     Volume:  15     ISSN:  0100-879X     ISO Abbreviation:  Braz. J. Med. Biol. Res.     Publication Date:  1982 Oct 
Date Detail:
Created Date:  1983-07-08     Completed Date:  1983-07-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8112917     Medline TA:  Braz J Med Biol Res     Country:  BRAZIL    
Other Details:
Languages:  eng     Pagination:  239-45     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / enzymology*
Chromatography
Cysteine Endopeptidases*
Electrophoresis, Paper
Endopeptidases / metabolism*
Glycine / analogs & derivatives*,  analysis
Gonadotropin-Releasing Hormone / antagonists & inhibitors*
Hydrolysis
Proline Oxidase / biosynthesis
Rabbits
Chemical
Reg. No./Substance:
33515-09-2/Gonadotropin-Releasing Hormone; 56-40-6/Glycine; 598-41-4/glycine amide; EC 1.5.3.-/Proline Oxidase; EC 3.4.-/Endopeptidases; EC 3.4.22.-/Cysteine Endopeptidases; EC 3.4.22.-/endopeptidase B

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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