Document Detail

Brain-derived neurotropic factor prevents superoxide anion-induced death of PC12h cells stably expressing TrkB receptor via modulation of reactive oxygen species.
MedLine Citation:
PMID:  10555159     Owner:  NLM     Status:  MEDLINE    
In our previous report (Satoh et al., 1999. Regulation of reactive oxygen species by nerve growth factor but not by Bcl-2 as a novel mechanism of protection of PC12 cells from superoxide anion-induced death. J. Biochem. 125, 952-959), we reported that nerve growth factor (NGF) protected PC12 cells from superoxide anion (O2-)-induced cell death through a novel regulation of reactive oxygen species (ROS) which increased O2- and decreased hydrogen peroxide (H2O2), indicating that decreasing conversion from O2- to H2O2 is a critical process for the protection by NGF. In the present study, we performed a comparative study on protective mechanisms between NGF and brain-derived neurotrophic factor (BDNF) using TrkB-expressing PC12h cells. When compared with NGF, BDNF induced a weaker but significant protective effect on the cells from O2- induced death. BDNF did not seem to change the total amount of ROS in the cells treated with xanthine and xanthine oxidase. On the other hand, BDNF increased O2- and decreased H2O2- levels in the same cells, although not so strongly as NGF. These results suggest that decreasing conversion from O2- to H2O2 is also critical for the protection by BDNF, which is considered to play a central role in survival and differentiation of CNS neurons.
T Yamagata; T Satoh; Y Ishikawa; A Nakatani; M Yamada; T Ikeuchi; H Hatanaka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience research     Volume:  35     ISSN:  0168-0102     ISO Abbreviation:  Neurosci. Res.     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-12-02     Completed Date:  1999-12-02     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8500749     Medline TA:  Neurosci Res     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  9-17     Citation Subset:  IM    
Division of Protein Biosynthesis, Institute for Protein Research, Osaka University, Suita, Japan.
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MeSH Terms
Brain-Derived Neurotrophic Factor / pharmacology*,  physiology
Cell Death / drug effects
Cell Survival / drug effects
Fluorescent Dyes
Microscopy, Fluorescence
Nerve Growth Factors / pharmacology,  physiology
Neuroprotective Agents / pharmacology
PC12 Cells
Reactive Oxygen Species / physiology*
Receptor, trkB / physiology*
Recombinant Proteins / metabolism,  pharmacology
Superoxides / metabolism*
Xanthine / toxicity
Xanthine Oxidase / toxicity
Reg. No./Substance:
0/2.5S nerve growth factor; 0/Brain-Derived Neurotrophic Factor; 0/Fluorescent Dyes; 0/Nerve Growth Factors; 0/Neuroprotective Agents; 0/Reactive Oxygen Species; 0/Recombinant Proteins; 11062-77-4/Superoxides; 69-89-6/Xanthine; EC Oxidase; EC, trkB

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