Document Detail


Brain-derived neurotrophic factor is upregulated in rats with chronic pancreatitis and mediates pain behavior.
MedLine Citation:
PMID:  21499209     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: We examined the role of brain-derived neurotrophic factor (BDNF) in the pathogenesis of pain in an experimental model of chronic pancreatitis (CP).
METHODS: Pancreatitis was induced by retrograde infusion of trinitrobenzene sulfonic acid into the pancreatic duct of adult rats. Twenty-one days after injection, BDNF expression was examined in pancreas-specific dorsal root ganglia (DRGs) by immunohistochemistry, and protein levels were quantified from DRGs and spinal cord extracts. The effects of intrathecal infusion of a neutralizing antibody to BDNF on pancreatic hyperalgesia were assessed by the sensitivity of the abdominal wall to filament probing as well as the nocifensive behavior to electrical stimulation of the pancreas.
RESULTS: Levels of BDNF in DRGs and spinal cords (T9-13) were significantly higher in trinitrobenzene sulfonic acid rats compared with controls, accompanied by an increase in the number of pancreas-specific neurons expressing BDNF immunoreactivity. Brain-derived neurotrophic factor antagonism suppressed phospho-tropomyosin-related kinase B receptor levels in the spinal cord and significantly reduced behavioral responses in rats with CP.
CONCLUSIONS: Brain-derived neurotrophic factor is upregulated in pancreas-specific primary afferent neurons in rats with CP, and BDNF antagonism is associated with a reduction of pain-related behavior in these animals, suggesting an important role for this neurotransmitter in the nociception of CP.
Authors:
Michael S Hughes; Mohan Shenoy; Liansheng Liu; Tugba Colak; Kshama Mehta; Pankaj J Pasricha
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Pancreas     Volume:  40     ISSN:  1536-4828     ISO Abbreviation:  Pancreas     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-04-18     Completed Date:  2011-08-01     Revised Date:  2014-07-10    
Medline Journal Info:
Nlm Unique ID:  8608542     Medline TA:  Pancreas     Country:  United States    
Other Details:
Languages:  eng     Pagination:  551-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies / immunology,  pharmacology
Behavior, Animal / drug effects
Blotting, Western
Brain-Derived Neurotrophic Factor / antagonists & inhibitors,  immunology,  metabolism*
Ganglia, Spinal / metabolism,  physiopathology
Immunohistochemistry
Male
Neurons / metabolism
Pain / physiopathology*,  prevention & control
Pain Measurement / methods
Pancreatitis, Chronic / chemically induced,  metabolism*,  physiopathology*
Phosphorylation / drug effects
Rats
Rats, Sprague-Dawley
Receptor, trkB / metabolism
Spinal Cord / metabolism
Trinitrobenzenesulfonic Acid
Up-Regulation / drug effects
Grant Support
ID/Acronym/Agency:
P30 DK56339/DK/NIDDK NIH HHS; R01 DK073558/DK/NIDDK NIH HHS; R01 DK073558/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies; 0/Brain-Derived Neurotrophic Factor; 8T3HQG2ZC4/Trinitrobenzenesulfonic Acid; EC 2.7.10.1/Receptor, trkB

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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