Document Detail


Brain area, age and viral vector-specific glial cell-line-derived neurotrophic factor expression and transport in rat.
MedLine Citation:
PMID:  17515788     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the feasibility of viral vector-mediated expression and axonal transport of the glial cell-line-derived neurotrophic factor, a potential antiepileptic agent, to the hippocampus and the piriform cortex, areas involved in the induction and spread of seizure activity. Glial cell-line-derived neurotrophic factor overexpression was induced by injections of recombinant vectors derived from serotype 2 adeno-associated virus or lentivirus. We found that recombinant adeno-associated viral vector was able to effectively transduce mitral cells of the olfactory bulb and pyramidal cells of CA1, resulting in transport of glial cell-line-derived neurotrophic factor to the piriform cortex and to the contralateral CA1 area, respectively. These data suggest that the recombinant adeno-associated viral vector vector system is an optimal alternative for therapeutic glial cell-line-derived neurotrophic factor gene transduction and transport of the protein to the epileptogenic brain areas.
Authors:
Irene Kanter-Schlifke; Biljana Georgievska; Deniz Kirik; Merab Kokaia
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroreport     Volume:  18     ISSN:  0959-4965     ISO Abbreviation:  Neuroreport     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-22     Completed Date:  2007-07-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9100935     Medline TA:  Neuroreport     Country:  England    
Other Details:
Languages:  eng     Pagination:  845-50     Citation Subset:  IM    
Affiliation:
Experimental Epilepsy Group, Section for Neuroscience, Department of Experimental Medical Science, Wallenberg Neuroscience Center, BMC A-11, Lund University, Lund, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae / genetics
Aging / physiology*
Animals
Animals, Newborn
Axonal Transport / physiology*
Brain / physiology*
Cytoplasmic Vesicles / metabolism
Genetic Vectors
Glial Cell Line-Derived Neurotrophic Factor / biosynthesis*,  genetics*,  metabolism
Green Fluorescent Proteins / biosynthesis,  genetics
Immunohistochemistry
Lentivirus / genetics
Male
Olfactory Bulb / cytology,  metabolism
Rats
Rats, Sprague-Dawley
Chemical
Reg. No./Substance:
0/Glial Cell Line-Derived Neurotrophic Factor; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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