Document Detail

Brain-derived neurotrophic factor-transfected and nontransfected 3T3 fibroblasts enhance migratory neuroblasts and functional restoration in mice with intracerebral hemorrhage.
MedLine Citation:
PMID:  23147508     Owner:  NLM     Status:  MEDLINE    
Neurogenesis via the activation of endogenous neural progenitor cells is a potential treatment strategy for brain injury, including intracerebral hemorrhage (ICH). We assessed the efficacy of combined cell and brain-derived neurotrophic factor (BDNF) treatment in a mouse model of ICH induced by intracerebral collagenase injection. Complementary DNAs of mouse BDNF were transfected into cell lines of 3T3 fibroblasts. The expression and bioactivity of BDNF were analyzed by immunocytochemistry, Western blot, ELISA, and functional assays. Hematoma area and brain tissue loss were assessed by magnetic resonance imaging. The BDNF-transfected or nontransfected 3T3 fibroblasts were implanted as a growth factor source in mice with ICH. Neurogenesis and functional recovery were evaluated 15 days after ICH. The BDNF-treated mice had the most doublecortin-positive cells near lesions and the least brain tissue loss in all groups. Both cell treatment groups had abundant newly proliferative glial fibrillary acidic protein-positive cells and better functional improvement than controls. These results indicate that fibroblast transplantation, together with recombinant BDNF treatment, after ICH is beneficial in mice. The early functional recovery may result from the growth factors that are provided or evoked by the implanted grafts. These results suggest a potential approach for combining gene and cell therapy for ICH treatment.
Shiu-Jau Chen; Jui-Chang Tsai; Tsung-Yi Lin; Chung-Yi Lin; Cheng-Kuei Chang; Tai-Hsiang Tseng; Chung-Liang Chien
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neuropathology and experimental neurology     Volume:  71     ISSN:  1554-6578     ISO Abbreviation:  J. Neuropathol. Exp. Neurol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-30     Completed Date:  2013-02-04     Revised Date:  2013-04-10    
Medline Journal Info:
Nlm Unique ID:  2985192R     Medline TA:  J Neuropathol Exp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1123-36     Citation Subset:  IM    
Department of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei, Taiwan.
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MeSH Terms
Analysis of Variance
BALB 3T3 Cells
Brain-Derived Neurotrophic Factor / genetics,  metabolism*
Cell Count
Cell Movement / physiology*
Cell Transplantation / methods*
Cerebral Hemorrhage / surgery*
Culture Media, Conditioned / chemistry,  pharmacology
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Fibroblasts / transplantation*
Green Fluorescent Proteins / genetics,  metabolism
Magnetic Resonance Imaging
Motor Activity / physiology
Neurologic Examination
Reg. No./Substance:
0/Brain-Derived Neurotrophic Factor; 0/Culture Media, Conditioned; 0/enhanced green fluorescent protein; 147336-22-9/Green Fluorescent Proteins
Erratum In:
J Neuropathol Exp Neurol. 2013 Mar;72(3):269
Note: Lin, Chung-Yi [corrected to Lin, Tsung-Yi]

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