| Bradykinin- and sodium nitroprusside-induced increases in capillary tube haematocrit in mouse cremaster muscle are associated with impaired glycocalyx barrier properties. | |
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MedLine Citation:
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PMID: 18450777 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous studies have suggested that agonists may increase functionally perfused capillary volume by modulation of blood-excluding glycocalyx volume, but direct evidence for this association is lacking at the moment. Using intravital microscopic visualization of mouse cremaster muscle, we determined the effects of bradykinin (10(-5) M) and sodium nitroprusside (10(-6) M) on capillary tube haematocrit and glycocalyx barrier properties. In control C57Bl/6 mice (n = 10), tube haematocrit in capillaries (n = 71) increased (P < 0.05) from 8.7 +/- 0.3% during baseline to 21.2 +/- 1.2 and 22.2 +/- 0.9% during superfusion with bradykinin and nitroprusside, respectively. In parallel, the exclusion zone of FITC-labelled 70 kDa dextrans decreased (P < 0.05) from 0.37 +/- 0.01 microm during baseline to 0.17 +/- 0.01 microm with bradykinin and 0.15 +/- 0.01 microm with nitroprusside. Bradykinin and nitroprusside had no effect on dextran exclusion and tube haematocrit in capillaries (n = 55) of hyperlipidemic ApoE3-Leiden mice, which showed impaired exclusion of 70 kDa dextrans (0.05 +/- 0.02 microm; P < 0.05 versus C57Bl/6) and increased capillary tube haematocrit (23 +/- 0.8%; P < 0.05 versus C57Bl/6) under baseline conditions, indicating glycocalyx degradation. Our data show that vasodilator substances increase functionally perfused capillary volume and that this effect is associated with a reduction in glycocalyx exclusion of 70 kDa dextrans. Modulation of glycocalyx volume might represent a novel mechanism of perfusion control at the capillary level. |
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Authors:
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Jurgen W G E VanTeeffelen; Alina A Constantinescu; Judith Brands; Jos A E Spaan; Hans Vink |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-05-01 |
Journal Detail:
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Title: The Journal of physiology Volume: 586 ISSN: 1469-7793 ISO Abbreviation: J. Physiol. (Lond.) Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-07-02 Completed Date: 2008-11-13 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0266262 Medline TA: J Physiol Country: England |
Other Details:
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Languages: eng Pagination: 3207-18 Citation Subset: IM |
Affiliation:
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Department of Physiology, Cardiovascular Research Institute Maastricht, Maastricht University, PO Box 616, 6200 MD Maastricht, The Netherlands. j.vanteeffelen@fys.unimaas.nl |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bradykinin / pharmacology* Capillaries / drug effects*, physiology Glycocalyx / metabolism* Hematocrit Hyperlipidemias / metabolism Male Mice Mice, Inbred C57BL Microscopy, Video Muscle, Skeletal / metabolism* Nitroprusside / pharmacology* Permeability Vasodilator Agents / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Vasodilator Agents; 15078-28-1/Nitroprusside; 58-82-2/Bradykinin |
| Comments/Corrections | |
Comment In:
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J Physiol. 2008 Oct 1;586(Pt 19):4585-6
[PMID:
18703574
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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