| Bradykinin metabolism in rat hind limbs. | |
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MedLine Citation:
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PMID: 9721983 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The purpose of this study was to assess the capacity of perfused rat hind limbs, the majority of which is skeletal muscle, to inactivate bradykinin (BK), and to compare the BK degrading capacity of rat hind limbs with the BK degrading capacities of rat lung and liver. BK, with tritiated Pro2 and Pro3 residues, in an asanguinous salt solution was perfused for a single passage through skeletal muscle and other tissues in the hind legs of the rat over a concentration range of .0029 to 49.3 microM. Rat hind limbs had a large capacity to degrade BK and the system did not approach saturation, even at 49.3 microM. A least-squares linear regression analysis and extrapolation to zero concentration was used to obtain values for amounts of BK degraded and BK fragments formed. The amount of BK cleaved was 95% of the administered dose. The major BK fragments formed, and the amount of each expressed as a percentage of the amount of BK degraded were Pro-Pro (BK 2-3), 8.6%; Arg-Pro-Pro-Gly-Phe (BK 1-5), 82%; and Arg-Pro-Pro-Gly-Phe-Ser-Pro (BK 1-7), 6%. The BK 1-5 yield was reduced from 82% to one-fourth of that by angiotensin converting enzyme (ACE) inhibitors. BK 2-3 formation is indicative of initial aminopeptidase-P cleavage of BK to yield Arg, and des-Arg1-BK. ACE inhibitor sensitive formation of BK 1-5 is indicative of initial kininase-II, also known as ACE, cleavage of BK. Thus in rat hind limbs, the ACE pathway is the preponderant mechanism for BK degradation, which is in contrast to our previously published reports that in rat liver the amino-peptidase-P pathway predominates, and that in rat lung both the aminopeptidase-P pathway and the ACE pathway exhibit nearly equal capacities to degrade BK. |
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Authors:
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J A Griswold; C R Baker; D T Little; G H Little; F J Behal |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Shock (Augusta, Ga.) Volume: 10 ISSN: 1073-2322 ISO Abbreviation: Shock Publication Date: 1998 Aug |
Date Detail:
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Created Date: 1998-11-09 Completed Date: 1998-11-09 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9421564 Medline TA: Shock Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 146-52 Citation Subset: IM |
Affiliation:
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Department of Surgery, Texas Tech University School of Medicine, Lubbock 79430, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bradykinin / analysis, chemistry, metabolism* Dipeptides / analysis Hindlimb Kinetics Liver / metabolism Lung / metabolism Male Muscle, Skeletal / blood supply, metabolism* Peptide Fragments / analysis Perfusion Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Dipeptides; 0/Peptide Fragments; 23815-89-6/bradykinin (1-5); 58-82-2/Bradykinin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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