| Bradykinin metabolism in perfused rat kidney. | |
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MedLine Citation:
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PMID: 10353545 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The purpose of this study was to assess the capacity of perfused rat kidney to inactivate bradykinin (BK), and to compare the BK degrading capacity of rat kidney with the BK degrading capacities of rat lung, liver, and skeletal muscle, which was approximated by perfusion of rat hind limbs. Radioactively labeled BK, with the Pro2 and Pro3 residues having been tritiated, in an asanguinous salt solution was perfused through the kidney of the rat, over a concentration range of .0028-33 microM. Rat kidney had a large capacity to degrade BK and the system did not appear to approach saturation until perfusate BK concentrations reached 24 microM. A least-squares linear regression analysis and extrapolation to zero concentration was used to obtain values for amounts of BK degraded and BK fragments formed. The amount of BK cleaved was 99.9% of the administered dose. The major tritiated BK fragments formed, and the amount of each expressed as a percentage of the amount of BK degraded during transrenal passage, were the amino acid proline derived from the Pro2 and/or Pro3 residues of BK (Pro2,3), 60%; Pro-Pro (BK 2-3), 12%; Arg-Pro-Pro-Gly-Phe (BK 1-5), 14%; and Arg-Pro-Pro-Gly-Phe-Ser-Pro (BK 1-7), 14%. The formation of BK 2-3 is indicative of initial aminopeptidase-P cleavage of BK to yield Arg, and des-Arg1-BK. Thus in rat kidney the aminopeptidase-P pathway is the major route for BK degradation, as is the case in rat liver. |
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Authors:
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J A Griswold; C R Baker; D T Little; G H Little; F J Behal |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Shock (Augusta, Ga.) Volume: 11 ISSN: 1073-2322 ISO Abbreviation: Shock Publication Date: 1999 May |
Date Detail:
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Created Date: 1999-08-03 Completed Date: 1999-08-03 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9421564 Medline TA: Shock Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 372-9 Citation Subset: IM |
Affiliation:
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Department of Surgery, Texas Tech University School of Medicine, Lubbock 79430, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bradykinin / metabolism* Kidney / metabolism* Linear Models Liver / metabolism Lung / metabolism Male Muscle, Skeletal / metabolism Perfusion Rats Rats, Sprague-Dawley Sequence Analysis |
| Chemical | |
Reg. No./Substance:
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58-82-2/Bradykinin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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