| Bradykinin-induced Ca2+ entry, release, and refilling of intracellular Ca2+ stores. Relationships revealed by image analysis of individual human fibroblasts. | |
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MedLine Citation:
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PMID: 1730576 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lys-Bradykinin (BK), a mitogen for human foreskin fibroblasts (HSWP cells) (Owen, N. E., and Villereal, M. L. (1983) Cell 32, 979-985), elicits a rapid, transient elevation of intracellular free Ca2+ concentration ([Ca2+]i) in these cells. We have used image analysis of fura-2-loaded HSWP cells to examine the BK-induced [Ca2+]i changes in individual cells. BK-stimulated Ca2+ entry and release of intracellular Ca2+ stores can be distinguished by stimulating cells in the presence or absence of extracellular Ca2+, or by inhibiting Ca2+ entry with 5 mM NiCl2. BK-sensitive intracellular Ca2+ stores can be depleted by exposure of the cells to BK in Ca(2+)-free medium; refilling of the stores requires extracellular Ca2+. A component of BK-stimulated Ca2+ entry persists after removal of agonist, but inactivates with a t1/2 of approximately 5 min. Although previous studies have attributed the Ca2+ entry which persists after agonist removal to a "capacitative Ca2+ entry" pathway activated by the depletion of the intracellular Ca2+ stores, we find that a large component of this BK-stimulated Ca2+ entry is not due to capacitative Ca2+ entry since (1) ionomycin can deplete the BK-sensitive intracellular Ca2+ stores without appreciably stimulating Ca2+ entry and without inhibiting the BK-stimulated Ca2+ entry and (2) this Ca2+ entry pathway inactivates at a time when the Ca2+ pools are still empty and a capacitance entry pathway should still be open. On the other hand, refilling of the intracellular Ca2+ stores can occur after the noncapacitative Ca2+ entry component has inactivated or when it is inhibited by Ni2+; in these cases refilling occurs without a detectable elevation of [Ca2+]i suggesting that refilling of internal Ca2+ pools might occur by a capacitative route. |
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Authors:
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K L Byron; G Babnigg; M L Villereal |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 267 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1992 Jan |
Date Detail:
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Created Date: 1992-02-18 Completed Date: 1992-02-18 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 108-18 Citation Subset: IM |
Affiliation:
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Department of Pharmacological and Physiological Sciences, University of Chicago, Illinois 60637. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biological Transport
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drug effects Bradykinin / pharmacology* Calcium / metabolism* Cell Line Fibroblasts / drug effects, metabolism Fura-2 Humans Image Processing, Computer-Assisted Inositol Phosphates / metabolism Ionomycin / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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5T32-GM-07151/GM/NIGMS NIH HHS; GM-28359/GM/NIGMS NIH HHS; S10-RR-03322/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Inositol Phosphates; 56092-81-0/Ionomycin; 58-82-2/Bradykinin; 7440-70-2/Calcium; 96314-98-6/Fura-2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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