Document Detail

Bradykinin generation in RC-MAP during storage at 4 degrees C and leukocyte removal filtration.
MedLine Citation:
PMID:  9330433     Owner:  NLM     Status:  MEDLINE    
We investigated contact system activation in red cell concentrate in mannitol-adenine-phosphate (MAP) solution (RC-MAP) during storage and leukocyte removal filtration. The contact system activation was assessed in terms of bradykinin (BK) generation. The BK level in the RC-MAP transiently but significantly (p < 0.05) increased at 7 to 14 days of storage. Moreover, the BK level in 21-day-stored RC-MAP was approximately 10 times higher than that in other blood components. The BK level in mannitol-containing MAP solutions, but not that in mannitol-free MAP solution, saline or whole blood, increased during storage. The plasma angiotensin-converting enzyme (ACE) activity decreased significantly only in mannitol-containing MAP solutions. These findings suggest that mannitol plays an essential role in the increase in BK level in RC-MAP. Furthermore, the BK level in RC-MAP increased 10 minutes after the start of filtration through a negatively charged filter, either a BPF-4 or an RN-40, and reached a maximum of 6,000 pg/ml. Hypotensive reactions are rare in RC-MAP transfusion in comparison to their incidence in platelet concentrate (PC) transfusion in spite of the higher BK level in RC-MAP than in PC. Therefore, BK generation in various blood components is not likely to be the main cause of hypotensive reactions. However, the level of BK in RC-MAP is sufficiently high to cause site pain. Further investigation on the clinical significance of a high BK level in blood components is necessary.
M Shiba; K Tadokoro; K Nakajima; T Juji
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Thrombosis research     Volume:  87     ISSN:  0049-3848     ISO Abbreviation:  Thromb. Res.     Publication Date:  1997 Sep 
Date Detail:
Created Date:  1997-11-25     Completed Date:  1997-11-25     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0326377     Medline TA:  Thromb Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  511-20     Citation Subset:  IM    
Japanese Red Cross Central Blood Center, Tokyo, Japan.
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MeSH Terms
Adenine / pharmacology*
Blood Preservation / methods*
Bradykinin / blood*,  drug effects
Erythrocytes / chemistry,  drug effects,  physiology
Mannitol / pharmacology*
Time Factors
Reg. No./Substance:
0/MAP solution; 58-82-2/Bradykinin; 69-65-8/Mannitol; 73-24-5/Adenine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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