Document Detail


Bradykinin effects on phospholipid metabolism and its relation to arachidonic acid turnover in neuroblastoma x glioma hybrid cells (NG 108-15).
MedLine Citation:
PMID:  2518358     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In neuroblastoma x glioma hybrid cells (NG 108-15) labelled with [32P]-trisodium phosphate, [3H]-inositol and [14C]-arachidonic acid, bradykinin stimulated the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) while it had no effect on the release of [14C]-arachidonic acid (AA). The effect on PIP2 was time- and dose-dependent with a maximal effect on [3H]-inositol- and [32P]-labelled cells after 10-30 s of stimulation with 10(-6) M bradykinin. However, the hydrolysis of [14C]-AA labelled PIP2 was delayed compared to the effect on [3H]- and [14C]-PIP2 and was not detectable until after 60 s of stimulation. Bradykinin stimulation resulted in an increased formation of [3H]-inositol phosphates (IP) and [32P]- and [14C]-phosphatidic acid (PA) but the time course for PA formation did not follow the time-course for PIP2 hydrolysis. A reduced labelling of [32P]- and [14C]-phosphatidylcholine was also found in stimulated cells suggesting that PA may derive from other sources than PIP2. In conclusion, our results indicate that bradykinin activates phospholipase C, but not phospholipase A2, in NG 108-15 cells.
Authors:
P Simonsson; G Y Sun; S Aradottir; C Alling
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular signalling     Volume:  1     ISSN:  0898-6568     ISO Abbreviation:  Cell. Signal.     Publication Date:  1989  
Date Detail:
Created Date:  1990-10-18     Completed Date:  1990-10-18     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8904683     Medline TA:  Cell Signal     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  587-98     Citation Subset:  IM    
Affiliation:
Department of Psychiatry and Neurochemistry, Lund University, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arachidonic Acid
Arachidonic Acids / metabolism*
Bradykinin / physiology*
Glioma
Hybrid Cells
Neuroblastoma
Neurons / metabolism*
Phosphatidylinositols / metabolism
Phospholipases A / physiology
Phospholipases A2
Phospholipids / metabolism*
Tumor Cells, Cultured
Type C Phospholipases / physiology
Chemical
Reg. No./Substance:
0/Arachidonic Acids; 0/Phosphatidylinositols; 0/Phospholipids; 506-32-1/Arachidonic Acid; 58-82-2/Bradykinin; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2; EC 3.1.4.-/Type C Phospholipases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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