Document Detail


Bovine serum albumin enhances nicotinic acetylcholine receptor function in mouse thalamic synaptosomes.
MedLine Citation:
PMID:  12358728     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bovine serum albumin (BSA) enhances nicotinic agonist-induced (86)Rb+ efflux from synaptosomal fractions of the mouse thalamus, but how it does so is not understood. The experiments reported here indicated that BSA enhancement of nicotinic acetylcholine receptor function was rapid, reversible, depended on BSA concentration, and occurred at all points of the nicotinic agonist concentration-response curve. We hypothesized that BSA-extractable compounds, such as long-chain fatty acids, were responsible for inhibiting nicotinic responses in the absence of BSA. The hypothesis was tested by applying arachidonic, linolenic, or oleic acids in the absence of BSA after an initial prewash with BSA. All three fatty acids exhibited a rapid, concentration-dependent inhibition of nicotinic-agonist stimulated ion flux. Concentration-response curves produced after 30 s of pre-treatment with arachidonic acid were similar to those seen when BSA was completely absent. The effects of pre-treatment were reversed immediately by the introduction of BSA. Furthermore, no effects of fatty acids were observed when preparations were continuously exposed to BSA or when BSA was continuously absent. These results suggest that the removal of endogenous, inhibitory compounds is largely responsible for the rapid, potentiating action of BSA at nicotinic acetylcholine receptors expressed in the mouse thalamus.
Authors:
Christopher M Butt; Scott R Hutton; Michael J Marks; Allan C Collins
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  83     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-10-02     Completed Date:  2002-10-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  48-56     Citation Subset:  IM    
Affiliation:
Institute for Behavioral Genetics, University of Colorado, Boulder Campus Box 447, Boulder, CO 80309, USA. Chris.Butt@colorado.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Arachidonic Acid / pharmacology
Azetidines / pharmacology
Dose-Response Relationship, Drug
Fatty Acids / pharmacology
Female
Male
Mice
Mice, Inbred C57BL
Nicotinic Agonists / pharmacology
Receptors, Nicotinic / drug effects*,  metabolism*
Rubidium / metabolism,  pharmacokinetics
Serum Albumin, Bovine / pharmacology*
Synaptosomes / chemistry,  drug effects,  metabolism*
Thalamus / chemistry*
Grant Support
ID/Acronym/Agency:
5T32 AA 07464/AA/NIAAA NIH HHS; AA 11156/AA/NIAAA NIH HHS; DA 00197/DA/NIDA NIH HHS
Chemical
Reg. No./Substance:
0/A 85380; 0/Azetidines; 0/Fatty Acids; 0/Nicotinic Agonists; 0/Receptors, Nicotinic; 0/Serum Albumin, Bovine; 506-32-1/Arachidonic Acid; 7440-17-7/Rubidium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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