Document Detail


Bovine proacrosin from cauda epididymal sperm: purification, characterization and partial sequencing at N-terminus.
MedLine Citation:
PMID:  1473607     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. In the present study, we isolated the two forms of proacrosin from acid extracts (pH 3.0) of cauda epididymal bovine spermatozoa by ammonium sulfate fractionation, gel filtration on Sephadex G-150 and affinity chromatography on Concanavalin A Sepharose 4B. The overall purification was 13-fold with respect to crude acid acrosomal extract. 2. The apparent molecular weight of the proacrosins determined by SDS-PAGE were 44,000 and 38,000. Both forms have proteinase activity on gelatin-SDS-polyacrylamide gel electrophoretic zymography. 3. The M(r) = 38,000 component was isolated by reverse phase HPLC. Thirty-nine amino acid residues at the N-terminus have about 72 and 77% sequence similarity with boar and human proacrosin, respectively. 4. The amino acid sequence of 14 amino acids at the N-terminus of the high molecular weight component (M(r) = 44,000) was determined after electroblotting on a polyvinylidene difluoride membrane. This portion of the molecule is identical with that of the low molecular weight component. 5. Proacrosin autoactivation followed the sigmoidal activation curve.
Authors:
S K NagDas
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The International journal of biochemistry     Volume:  24     ISSN:  0020-711X     ISO Abbreviation:  Int. J. Biochem.     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1993-01-29     Completed Date:  1993-01-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0250365     Medline TA:  Int J Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1943-52     Citation Subset:  IM    
Affiliation:
Institute for Enzyme Research, University of Wisconsin, Madison 53705.
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MeSH Terms
Descriptor/Qualifier:
Acrosin / genetics,  isolation & purification*,  metabolism
Amino Acid Sequence
Animals
Calcium / pharmacology
Cattle
Enzyme Activation
Enzyme Precursors / genetics,  isolation & purification*,  metabolism
Epididymis / enzymology
Humans
Kinetics
Male
Molecular Sequence Data
Molecular Weight
Sequence Homology, Amino Acid
Species Specificity
Spermatozoa / enzymology*
Swine
Grant Support
ID/Acronym/Agency:
AM 10334/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Enzyme Precursors; 7440-70-2/Calcium; EC 3.4.21.-/proacrosin; EC 3.4.21.10/Acrosin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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