Document Detail

Bovine proacrosin from cauda epididymal sperm: purification, characterization and partial sequencing at N-terminus.
MedLine Citation:
PMID:  1473607     Owner:  NLM     Status:  MEDLINE    
1. In the present study, we isolated the two forms of proacrosin from acid extracts (pH 3.0) of cauda epididymal bovine spermatozoa by ammonium sulfate fractionation, gel filtration on Sephadex G-150 and affinity chromatography on Concanavalin A Sepharose 4B. The overall purification was 13-fold with respect to crude acid acrosomal extract. 2. The apparent molecular weight of the proacrosins determined by SDS-PAGE were 44,000 and 38,000. Both forms have proteinase activity on gelatin-SDS-polyacrylamide gel electrophoretic zymography. 3. The M(r) = 38,000 component was isolated by reverse phase HPLC. Thirty-nine amino acid residues at the N-terminus have about 72 and 77% sequence similarity with boar and human proacrosin, respectively. 4. The amino acid sequence of 14 amino acids at the N-terminus of the high molecular weight component (M(r) = 44,000) was determined after electroblotting on a polyvinylidene difluoride membrane. This portion of the molecule is identical with that of the low molecular weight component. 5. Proacrosin autoactivation followed the sigmoidal activation curve.
S K NagDas
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The International journal of biochemistry     Volume:  24     ISSN:  0020-711X     ISO Abbreviation:  Int. J. Biochem.     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1993-01-29     Completed Date:  1993-01-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0250365     Medline TA:  Int J Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1943-52     Citation Subset:  IM    
Institute for Enzyme Research, University of Wisconsin, Madison 53705.
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MeSH Terms
Acrosin / genetics,  isolation & purification*,  metabolism
Amino Acid Sequence
Calcium / pharmacology
Enzyme Activation
Enzyme Precursors / genetics,  isolation & purification*,  metabolism
Epididymis / enzymology
Molecular Sequence Data
Molecular Weight
Sequence Homology, Amino Acid
Species Specificity
Spermatozoa / enzymology*
Grant Support
Reg. No./Substance:
0/Enzyme Precursors; 7440-70-2/Calcium; EC 3.4.21.-/proacrosin; EC

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