Document Detail

Bovine oocytes cryoinjury and how to improve their development following cryopreservation.
MedLine Citation:
PMID:  23534957     Owner:  NLM     Status:  In-Data-Review    
Bovine oocytes are less likely to undergo successful cryopreservation than cleavage-stage embryos. Bovine oocytes characteristically contain high levels of lipids that represent one of the major obstacles limiting efficient cryopreservation. These droplets together with structures such as cumulus cells, zona pellucida, cytoplasm membrane, cortical granules, mitochondria, spindle, and cytoskeleton (microtubles and microfilaments) often incur serious damage during cooling and warming. The cryoinjury could, to some extent, be decreased by selection of proper permeable and non-permeable cryoprotectants, and of vitrification with high cooling and warming rates. Additionally, such measures may also enhance their cryotolerance as partial removal of cumulus cells, modification of oocyte membrane constituents, polarization of the cytoplasmic lipid droplets by centrifugation, and addition of cytoskeleton relaxants or ice blockers into vitrification solutions. The improvement in cryopreservation methodology for bovine oocytes will no doubt augment other technologies such as bovine cloning and the establishment of gene bank for transgenic cattle.
Guang Bin Zhou; Ning Li
Related Documents :
24478357 - Activity of the principal cells of the olfactory bulb promotes a structural dynamic on ...
24939707 - Structural characterization and subcellular localization of drosophila organic solute c...
25125327 - Lysosomal capturing of cytoplasmic injected nanoparticles by autophagy: an additional b...
23124947 - Environmental regulation of mitochondria-rich cells in chalcalburnus tarichi (pallas, 1...
16567367 - Histamine uptake by human endometrial cells expressing the organic cation transporter e...
11976927 - Cell membrane destabilizes progressively during repetitive mechanical rupture events.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Animal biotechnology     Volume:  24     ISSN:  1532-2378     ISO Abbreviation:  Anim. Biotechnol.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9011409     Medline TA:  Anim Biotechnol     Country:  England    
Other Details:
Languages:  eng     Pagination:  94-106     Citation Subset:  IM    
a Institute of Animal Genetics and Breeding, College of Animal Science and Technology , Sichuan Agricultural University (Chengdu Campus) , Chengdu , P. R. China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  IGF1 mRNA Splicing Variants in Liaoning Cashmere Goat: Identification, Characterization, and Transcr...
Next Document:  Ammonia assimilation in rumen bacteria: a review.