Document Detail


Bovine oocytes cryoinjury and how to improve their development following cryopreservation.
MedLine Citation:
PMID:  23534957     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Bovine oocytes are less likely to undergo successful cryopreservation than cleavage-stage embryos. Bovine oocytes characteristically contain high levels of lipids that represent one of the major obstacles limiting efficient cryopreservation. These droplets together with structures such as cumulus cells, zona pellucida, cytoplasm membrane, cortical granules, mitochondria, spindle, and cytoskeleton (microtubles and microfilaments) often incur serious damage during cooling and warming. The cryoinjury could, to some extent, be decreased by selection of proper permeable and non-permeable cryoprotectants, and of vitrification with high cooling and warming rates. Additionally, such measures may also enhance their cryotolerance as partial removal of cumulus cells, modification of oocyte membrane constituents, polarization of the cytoplasmic lipid droplets by centrifugation, and addition of cytoskeleton relaxants or ice blockers into vitrification solutions. The improvement in cryopreservation methodology for bovine oocytes will no doubt augment other technologies such as bovine cloning and the establishment of gene bank for transgenic cattle.
Authors:
Guang Bin Zhou; Ning Li
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Animal biotechnology     Volume:  24     ISSN:  1532-2378     ISO Abbreviation:  Anim. Biotechnol.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9011409     Medline TA:  Anim Biotechnol     Country:  England    
Other Details:
Languages:  eng     Pagination:  94-106     Citation Subset:  IM    
Affiliation:
a Institute of Animal Genetics and Breeding, College of Animal Science and Technology , Sichuan Agricultural University (Chengdu Campus) , Chengdu , P. R. China.
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