Document Detail

Bovine leukemia virus alters growth properties and casein synthesis in mammary epithelial cells.
MedLine Citation:
PMID:  14507019     Owner:  NLM     Status:  MEDLINE    
Bovine leukemia virus (BLV) is widespread in US dairy herds, yet only about 1% of infected cattle develop bovine leukosis and are culled from the herd. A major concern is whether BLV infection of dairy cows alters milk yield. Although several studies have examined the effect of BLV on milk production in vivo, the results were inconclusive. No in vitro studies have been done. The discovery of BLV in mammary epithelial cells (MEC) of infected cows raises the possibility that the virus could affect these cells directly. The purpose of this study was to use an in vitro system to determine if BLV could alter milk yield by altering cell number and/or milk production per cell. A short-term cell line established from the MEC of a BLV-negative cow, and a proven casein-producer mouse cell line, Comma D, were stably transfected with a plasmid containing the entire BLV genome. Untransfected parental lines served as negative controls. The BLV-containing bovine MEC line has a reduced population-doubling time, higher saturation density, and increased longevity. The Comma D line is an already-transformed cell line, and growth properties did not change after transfection with BLV. Under appropriate differentiation conditions, both the bovine and mouse MEC transfected with BLV displayed decreased casein production and mRNA synthesis compared with control cell lines without BLV. Our results suggest that effects of BLV infection on milk production may not be related solely to overall animal health but may also be mediated directly at a cellular level.
D D Motton; G C Buehring
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of dairy science     Volume:  86     ISSN:  0022-0302     ISO Abbreviation:  J. Dairy Sci.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-09-24     Completed Date:  2003-11-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985126R     Medline TA:  J Dairy Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2826-38     Citation Subset:  IM    
Infectious Diseases Division, School of Public Health, University of California, Berkeley 94720, USA.
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MeSH Terms
Blotting, Western
Caseins / analysis,  biosynthesis*,  genetics
Cell Division*
Cell Line
Cell Line, Transformed
Enzyme-Linked Immunosorbent Assay
Epithelial Cells / cytology,  metabolism,  virology
Leukemia Virus, Bovine / genetics,  physiology*
Mammary Glands, Animal / cytology,  metabolism,  virology*
RNA, Messenger / analysis
Receptors, Estrogen / analysis
Receptors, Glucocorticoid / analysis
Receptors, Progesterone / analysis
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Caseins; 0/RNA, Messenger; 0/Receptors, Estrogen; 0/Receptors, Glucocorticoid; 0/Receptors, Progesterone

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