Document Detail


Bovine leukaemia virus-induced lymphocytosis in sheep is associated with reduction of spontaneous B cell apoptosis.
MedLine Citation:
PMID:  9010299     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Experimental inoculation of sheep with bovine leukaemia virus (BLV), a retrovirus homologous to the human T-lymphotropic virus type 1 (HTLV-1), induces a chronic expansion of the B lymphocyte population (persistent lymphocytosis) and the development of a B cell leukaemia/lymphosarcoma syndrome. To gain insight into the mechanisms of BLV-induced lymphocytosis, we tested B cell survival capacity and cycling activity in peripheral blood mononuclear cells (PBMCs) from lymphocytotic, asymptomatic and control sheep. Interestingly, B cells from lymphocytotic sheep presented a lower level of spontaneous apoptosis (29%) in ex vivo cultures compared to that obtained with infected asymptomatic (42%) and control (57%/o) sheep PBMCs. Virus capsid (CA) synthesis was mainly found among surviving B cells and the percentage of CA-producing B cells correlated with the extent of B cell survival, indicating that BLV replication in B lymphocytes may promote protection from cell death. B cell survival was not linked with increases in expression of Bcl-2 mRNA or membrane leukosialin (CD43), although both are documented to be involved in some aspects of the B cell life-span. Finally, cell cycle analyses in freshly isolated PBMCs from lymphocytotic sheep revealed a slightly increased proportion of B cells in S phase compared to controls. Altogether, these data suggest that both BLV-induced B cell proliferation and extended survival are involved in the lymphocytotic stage encountered in BLV infection in sheep.
Authors:
I Schwartz-Cornil; N Chevallier; C Belloc; D Le Rhun; V Lainé; M Berthelemy; A Mateo; D Levy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of general virology     Volume:  78 ( Pt 1)     ISSN:  0022-1317     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  1997 Jan 
Date Detail:
Created Date:  1997-02-24     Completed Date:  1997-02-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  153-62     Citation Subset:  IM; X    
Affiliation:
URA INRA d'Immuno Pathologie Cellulaire et Moléculaire, Maisons-Alfort, France. schwartz@jouy.inra.fr
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis*
B-Lymphocytes / cytology,  immunology*,  pathology
Cattle
Cell Cycle
Cell Survival
Cells, Cultured
Enzootic Bovine Leukosis / immunology*,  physiopathology
Flow Cytometry
Human T-lymphotropic virus 1
Humans
Leukemia Virus, Bovine*
Lymphocytosis / immunology*,  virology
Polymerase Chain Reaction
Proto-Oncogene Proteins c-bcl-2 / biosynthesis
RNA, Messenger
Reference Values
Sheep
Transcription, Genetic
Chemical
Reg. No./Substance:
0/Proto-Oncogene Proteins c-bcl-2; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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