Bovine lactoferrin induces interleukin-11 production in a hepatitis mouse model and human intestinal myofibroblasts. | |
MedLine Citation:
|
PMID: 21688123 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
PURPOSE: Orally administered bovine lactoferrin (bLF) exerts an anti-inflammatory effect on hepatitis and colitis animal models. To investigate the mechanism underlying the action of bLF, we explored the expression of inflammation-related factors in the intestine of a hepatitis mouse model after the oral administration of bLF and in several human intestinal cell lines treated with bLF. METHODS: The effects of bLF on the expression of interleukin-11 (IL-11) and bone morphogenetic protein 2 (BMP2) in the intestinal mucosa of a hepatitis mouse model as well as in cell cultures of human intestinal epithelial cells, myofibroblasts, and monocytes were examined using the real-time reverse transcription polymerase chain reaction. Epithelial cells and myofibroblasts were also cocultured using transwells. bLF transport, and IL-11 and BMP2 induction, as well as the interactions between the two cell types, were then analyzed after bLF treatment. RESULTS: In vivo, oral bLF administration increased the production of IL-11 and BMP2 in intestinal specimens. In vitro, bLF only stimulated the production of IL-11 in human intestinal myofibroblasts; i.e., it had no effect on BMP2 production in any cell type. In the transwell cocultures, bLF passed through the epithelium and directly stimulated IL-11 production in the myofibroblasts on the basolateral side. The IL-11 produced in the myofibroblasts subsequently acted protectively on the epithelial cells of the coculture. CONCLUSIONS: bLF upregulated the activity of anti-inflammatory factors, such as IL-11, in the intestine of a hepatitis mouse model and human intestinal myofibroblasts. |
Authors:
|
Tetsuya Kuhara; Koji Yamauchi; Keiji Iwatsuki |
Related Documents
:
|
21544493 - Thermotolerance and sensitivity of human cancer cells to cisplatin and doxorubicin. 2423353 - Cell cycle-dependent expression of nuclear matrix proteins of ehrlich ascites cells stu... 14724743 - Gap-junctional hemichannels are activated by atp depletion in human renal proximal tubu... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-6-19 |
Journal Detail:
|
Title: European journal of nutrition Volume: - ISSN: 1436-6215 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
|
Created Date: 2011-6-20 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100888704 Medline TA: Eur J Nutr Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Food Science & Technology Institute, Morinaga Milk Industry Co., Ltd., 5-1-83 Higashihara, Zama, Kanagawa, 228-8583, Japan, t_kuhara@morinagamilk.co.jp. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
MeSH Terms | |
Descriptor/Qualifier:
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The immunomodulatory properties of viable Lactobacillus salivarius ssp. salivarius CECT5713 are not ...
Next Document: The Protective Mechanism for the Blood-Brain Barrier Induced by Aminoguanidine in Surgical Brain Inj...