| Bovine herpesvirus-1 (infectious bovine rhinotracheitis virus)-based viral vector which expresses foot-and-mouth disease epitopes. | |
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MedLine Citation:
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PMID: 1722936 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A recombinant infectious bovine rhinotracheitis virus (IBRV) vector has been constructed to express bovine growth hormone signal sequence plus a foot-and-mouth disease virus [FMDV (O1K)] capsid protein (VP1) epitope as the N-terminal sequence of an IBRV glycoprotein gIII fusion protein on the surface of virus infected cells and on the surface of virus particles. Sequences encoding the first 38 amino acids of IBRV gIII were deleted from the recombinant to avoid redundant glycoprotein signal sequences, but IBRV gIII epitopes detected by anti-gIII monoclonal antibodies were retained. Phenotypes were confirmed by in situ immunostaining of virus plaques with anti-FMDV peptide sera, by immunogold staining of permeabilized- and non-permeabilized infected cells, and by virus neutralization experiments with anti-FMDV peptide sera. Vaccination with the IBRV-FMDV recombinant induced protective levels of anti-FMDV antibodies in calves and protected them from challenge with virulent IBRV. |
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Authors:
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M Kit; S Kit; S P Little; R D Di Marchi; C Gale |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Vaccine Volume: 9 ISSN: 0264-410X ISO Abbreviation: Vaccine Publication Date: 1991 Aug |
Date Detail:
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Created Date: 1992-02-24 Completed Date: 1992-02-24 Revised Date: 2006-04-21 |
Medline Journal Info:
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Nlm Unique ID: 8406899 Medline TA: Vaccine Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 564-72 Citation Subset: IM |
Affiliation:
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NovaGene Inc., Houston, TX. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Antibodies, Viral / biosynthesis* Antigens, Viral / immunology Aphthovirus / genetics, immunology* Base Sequence Blotting, Southern Capsid / genetics, immunology* Capsid Proteins Cattle Cell Line Epitopes Genetic Vectors Herpesvirus 1, Bovine / genetics*, immunology Immunization Infectious Bovine Rhinotracheitis / prevention & control Molecular Sequence Data Neutralization Tests Recombinant Fusion Proteins / immunology Staining and Labeling Vaccines, Synthetic / immunology Viral Proteins / immunology Viral Vaccines / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Viral; 0/Antigens, Viral; 0/Capsid Proteins; 0/Epitopes; 0/Recombinant Fusion Proteins; 0/VP1 protein, Foot-and-mouth disease virus; 0/Vaccines, Synthetic; 0/Viral Proteins; 0/Viral Vaccines; 0/bovine herpesvirus type-1 glycoproteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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