Document Detail

Bovine herpesvirus 1-induced apoptosis occurs at the G0/G1 phase of the cell cycle.
MedLine Citation:
PMID:  9191849     Owner:  NLM     Status:  MEDLINE    
We have previously shown that bovine herpesvirus 1 (BHV-1), even when inactivated, induces apoptotic cell death in mitogen-stimulated bovine peripheral blood mononuclear cells (PBMCs) (Hanon et al., 1996, J. Virol. 70, 4116-4120). In order to gain insight into this process, we have investigated the cell cycle phase at which BHV-1 induces apoptosis in PBMCs. Our results show that the percentage of cells that progress through the S phase was always lower in BHV-1-infected PBMCs than in control cells. This effect was not due to a defective activation of mitogen-stimulated PBMCs since BHV-1 only slightly affected the percentage of cells expressing BoCD25, a well-known lymphocyte activation marker. Furthermore, mimosine and cyclosporine A, two chemicals that inhibit entry into the S phase of the cell cycle by different pathways, did not affect the ability of BHV-1 to induce apoptosis. BHV-1-induced apoptosis also occurred in unstimulated PBMCs and interestingly, this was associated with the expression of c-myc and BoCD25 proteins both of which are related to cell cycle progression. All together, these data provide evidence demonstrating that BHV-1-induced apoptosis occurs at the G0/G1 phase of the cell cycle.
E Hanon; S Hoornaert; F Dequiedt; A Vanderplasschen; J Lyaku; L Willems; P P Pastoret
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virology     Volume:  232     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  1997 Jun 
Date Detail:
Created Date:  1997-07-15     Completed Date:  1997-07-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  351-8     Citation Subset:  IM    
Department of Immunology/Vaccinology, Faculty of Veterinary Medicine, University of Liège, Belgium.
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MeSH Terms
Cell Line
G0 Phase
G1 Phase
Herpesvirus 1, Bovine / physiology*
Leukocytes, Mononuclear / cytology*,  virology
Mitogens / pharmacology
Phorbol 12,13-Dibutyrate / pharmacology
Reg. No./Substance:
0/Mitogens; 37558-16-0/Phorbol 12,13-Dibutyrate

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