Document Detail


Bovine dentine organic matrix down-regulates osteoclast activity.
MedLine Citation:
PMID:  19296049     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Physiological root resorption is a phenomenon that normally takes place in deciduous teeth; root resorption of permanent teeth occurs only under pathological conditions. The molecular mechanisms underlying these processes are still unclear. Our previous study showed that osteoclasts cultured on deciduous dentine exhibited a higher degree of resorption and higher levels of cathepsin K and MMP-9 mRNA than osteoclasts cultured on permanent dentine. These results could be because of different susceptibilities to acid and the different organic matrices between deciduous and permanent dentine. Thus, the purpose of this study was to investigate the effect of dentine extracts from bovine deciduous and permanent dentine on osteoclast activity. Osteoclasts, obtained from mouse bone marrow cells co-cultured with an osteoblast-rich fraction in the presence of 1,25-(OH)(2)-vitamin D3 and PGE2, were incubated with or without 0.6 M HCl extracts from bovine deciduous or permanent dentine for 48 h. TRAP positive cell number, TRAP activity, the areas of resorption pits, and mRNA levels of TRAP, v-ATPase, calcitonin receptor, cathepsin K, and MMP-9 were examined. The results illustrated that TRAP activity, the resorbed area, and the mRNA levels of osteoclast marker genes seemed to be suppressed by both deciduous and permanent dentine extracts. These findings indicate that some factors that suppress osteoclast activity are contained in both deciduous and permanent dentine extracts. Although there was no significant difference in osteoclast activity between deciduous and permanent dentine extracts, osteoclasts incubated with permanent dentine extracts tend to exhibit less resorption activity than those incubated with deciduous dentine extracts. However, we could not clearly explain the causes of this.
Authors:
Wantida Sriarj; Kazuhiro Aoki; Keiichi Ohya; Yuzo Takagi; Hitoyata Shimokawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-20
Journal Detail:
Title:  Journal of bone and mineral metabolism     Volume:  27     ISSN:  0914-8779     ISO Abbreviation:  J. Bone Miner. Metab.     Publication Date:  2009  
Date Detail:
Created Date:  2009-04-16     Completed Date:  2009-06-17     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9436705     Medline TA:  J Bone Miner Metab     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  315-23     Citation Subset:  IM    
Affiliation:
Section of Developmental Oral Health Science, Department of Orofacial Development and Function, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / metabolism
Animals
Bone Resorption / metabolism
Cattle
Cell Count
Cell Differentiation / drug effects
Coculture Techniques
Dentin / metabolism*
Down-Regulation* / drug effects
Electrophoresis, Polyacrylamide Gel
Extracellular Matrix / drug effects,  metabolism*
Isoenzymes / metabolism
Mice
Osteoclasts / cytology*,  drug effects,  enzymology
RNA, Messenger / genetics,  metabolism
Tissue Extracts / pharmacology
src-Family Kinases / antagonists & inhibitors
Chemical
Reg. No./Substance:
0/Isoenzymes; 0/RNA, Messenger; 0/Tissue Extracts; EC 2.7.10.2/src-Family Kinases; EC 3.1.3.-/tartrate-resistant acid phosphatase; EC 3.1.3.2/Acid Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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