Document Detail

Bovine dentine organic matrix down-regulates osteoclast activity.
MedLine Citation:
PMID:  19296049     Owner:  NLM     Status:  MEDLINE    
Physiological root resorption is a phenomenon that normally takes place in deciduous teeth; root resorption of permanent teeth occurs only under pathological conditions. The molecular mechanisms underlying these processes are still unclear. Our previous study showed that osteoclasts cultured on deciduous dentine exhibited a higher degree of resorption and higher levels of cathepsin K and MMP-9 mRNA than osteoclasts cultured on permanent dentine. These results could be because of different susceptibilities to acid and the different organic matrices between deciduous and permanent dentine. Thus, the purpose of this study was to investigate the effect of dentine extracts from bovine deciduous and permanent dentine on osteoclast activity. Osteoclasts, obtained from mouse bone marrow cells co-cultured with an osteoblast-rich fraction in the presence of 1,25-(OH)(2)-vitamin D3 and PGE2, were incubated with or without 0.6 M HCl extracts from bovine deciduous or permanent dentine for 48 h. TRAP positive cell number, TRAP activity, the areas of resorption pits, and mRNA levels of TRAP, v-ATPase, calcitonin receptor, cathepsin K, and MMP-9 were examined. The results illustrated that TRAP activity, the resorbed area, and the mRNA levels of osteoclast marker genes seemed to be suppressed by both deciduous and permanent dentine extracts. These findings indicate that some factors that suppress osteoclast activity are contained in both deciduous and permanent dentine extracts. Although there was no significant difference in osteoclast activity between deciduous and permanent dentine extracts, osteoclasts incubated with permanent dentine extracts tend to exhibit less resorption activity than those incubated with deciduous dentine extracts. However, we could not clearly explain the causes of this.
Wantida Sriarj; Kazuhiro Aoki; Keiichi Ohya; Yuzo Takagi; Hitoyata Shimokawa
Related Documents :
23442259 - Percutaneous vertebroplasty combined with interstitial implantation of 125i seeds in ba...
16646639 - Sem-morphology in dentinogenesis imperfecta type ii: microscopic anatomy and efficacy o...
1133089 - On the configuration of incremental lines in human dentine as revealed by tetracycline ...
8639369 - Criteria for caries removal at the enamel-dentine junction: a clinical and microbiologi...
8771329 - Dimensional changes of demineralized human dentine during preparation for scanning elec...
20304479 - Effect of a new desensitizing material on human dentin permeability.
3140179 - An endostomal tracheostomy tube for leakproof retention of the blom-singer stomal valve.
10686869 - The effect of physiological root resorption on the histological structure of primary to...
10572619 - Hard, soft tissue and in vitro cell response to porous nickel-titanium: a biocompatibil...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-20
Journal Detail:
Title:  Journal of bone and mineral metabolism     Volume:  27     ISSN:  0914-8779     ISO Abbreviation:  J. Bone Miner. Metab.     Publication Date:  2009  
Date Detail:
Created Date:  2009-04-16     Completed Date:  2009-06-17     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9436705     Medline TA:  J Bone Miner Metab     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  315-23     Citation Subset:  IM    
Section of Developmental Oral Health Science, Department of Orofacial Development and Function, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Acid Phosphatase / metabolism
Bone Resorption / metabolism
Cell Count
Cell Differentiation / drug effects
Coculture Techniques
Dentin / metabolism*
Down-Regulation* / drug effects
Electrophoresis, Polyacrylamide Gel
Extracellular Matrix / drug effects,  metabolism*
Isoenzymes / metabolism
Osteoclasts / cytology*,  drug effects,  enzymology
RNA, Messenger / genetics,  metabolism
Tissue Extracts / pharmacology
src-Family Kinases / antagonists & inhibitors
Reg. No./Substance:
0/Isoenzymes; 0/RNA, Messenger; 0/Tissue Extracts; EC Kinases; EC 3.1.3.-/tartrate-resistant acid phosphatase; EC Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The effect and mechanism of action of capsaicin on gastric acid output.
Next Document:  Glomus jugulare tumours: certain clinical and radiological aspects observed following Gamma Knife ra...