| Bovine cumulus-oocyte-complex-quality is reflected in sensitivity for alpha-amanitin, oocyte-diameter and developmental capacity. | |
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MedLine Citation:
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PMID: 11182508 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of the present study was to find more parameters to define developmental competence of cumulus-oocyte-complexes (COCs). Bovine COCs were divided into five groups based on their morphology. In order of increasing level of atresia: COC-A had a bright, compact cumulus investment; COC-B1 also had a compact cumulus investment, but was darker than COC-A; the color of COC-B2 was comparable with COC-B1 but the corona radiata appeared to dissociate from the rest of the cumulus investment; the cumulus of COC-B3 was almost black and the corona radiata was almost completely dissociated from the rest of the cumulus investment; COC-C had a strongly expanded cumulus investment with dark spots of degenerated cells. An increasing level of atresia was accompanied by: (1) an increasing zona pellucida diameter (147.6, 150.8, 151.0, 154.3 and 155.1 microm, respectively, for COC-A, COC-B1, COC-B2, COC-B3 and COC-C); (2) an increasing oocyte diameter except for COC-C (120.9, 122.8, 122.8, 123.9 and 118.4 microm, respectively); (3) an increasing developmental competence except for COC-C (13.9, 14.7, 17.4, 19.1 and 11.5%, respectively, development to morula and blastocyst after in vitro embryo production (IVP)) and (4) by a increasing percentage of oocytes exhibiting germinal vesicle breakdown (GVBD) after 24h culture with alpha-amanitin and FSH, except for COC-A (34.4, 68.6, 51.0, 22.0 and 4.8%, respectively, oocytes arrested in GV). In general, embryo-quality, expressed in nuclei-count, was significantly affected by COC-quality (P < 0.05) with B3 > B1> B2 > A > C. However, if the developmental stages were compared separately, this effect was less evident. It was found that, in absence of FSH, alpha-amanitin was unable to inhibit GVBD and that the success of GVBD inhibition was positively correlated to the amount of cumulus cells surrounding the oocyte. COCs that had been exposed to alpha-amanitin and FSH during maturation retained the ability to cleave after IVF, but were unable to develop any further. |
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Authors:
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A A de Wit; T A Kruip |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Animal reproduction science Volume: 65 ISSN: 0378-4320 ISO Abbreviation: Anim. Reprod. Sci. Publication Date: 2001 Jan |
Date Detail:
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Created Date: 2001-02-22 Completed Date: 2001-05-24 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 7807205 Medline TA: Anim Reprod Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 51-65 Citation Subset: IM |
Affiliation:
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Institute for Animal Science and Health (ID-DLO), P.O. Box 65, 8200 AB, Lelystad, The Netherlands. a.a.c.dewit@id.dlo.nl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amanitins
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pharmacology* Animals Cattle / physiology* Coloring Agents Embryonic and Fetal Development / drug effects, physiology* Female Fertilization in Vitro / veterinary Follicle Stimulating Hormone / pharmacology Male Microscopy, Phase-Contrast / veterinary Nucleic Acid Synthesis Inhibitors / pharmacology* Oocytes / cytology, drug effects, physiology* Oxazines Regression Analysis Staining and Labeling Zona Pellucida / drug effects, physiology |
| Chemical | |
Reg. No./Substance:
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0/Amanitins; 0/Coloring Agents; 0/Nucleic Acid Synthesis Inhibitors; 0/Oxazines; 0/aceto-orcein; 9002-68-0/Follicle Stimulating Hormone |
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