| Bovine blastocysts with developmental competence to term share similar expression of developmentally important genes although derived from different culture environments. | |
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MedLine Citation:
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PMID: 21799070 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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This study was conducted to investigate the gene expression profile of in vivo derived bovine embryo biopsies based on pregnancy outcomes after transfer to recipients. For this, biopsies (30-40%) were taken from grade I blastocysts (IETS Manual) and the remaining 60-70% of the intact embryo were transferred to recipients. Frozen biopsies were pooled into three distinct groups based on the pregnancy outcome after transfer of the corresponding parts, namely: those resulting in No Pregnancy (NP), those resulting in Pregnancy Loss (PL) and those resulting in Calf Delivery. Array analysis revealed a total of 41 and 43 genes to be differentially expressed between biopsies derived from blastocysts resulting in No Pregnancy vs. Calf Delivery and Pregnancy Loss vs. Calf Delivery, respectively. Genes regulating nucleosome assembly (KRT8), placenta development and embryo maternal interaction (PLAC8) were found up-regulated in embryo biopsies which ended up with Calf Delivery. Embryo biopsies that failed to induce pregnancy were enriched with mitochondrial transcripts (Fl405) and stress related genes (HSPD1). Overall, gene expression profiles of blastocysts resulting in No Pregnancy and Calf Delivery group shared similar expression profiles with respect to genes playing significant roles in preimplantation embryo development. Finally, comparing the transcript signatures of in vivo and in vitro derived embryos with developmental competence to term revealed a similarity in the relative abundance of 18 genes. Therefore, we are able to present a genetic signature associated with term developmental competence independent to the environmental origin of the transferred blastocysts. |
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Authors:
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Nasser Ghanen; Dessie Salilew Wondim; Dawid Tesfaye; Ahmed Yehia Gad; Chirawath Phatsara; Ernst Tholen; Christian Looft; Karl Schellander; Michael Hoelker |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-7-28 |
Journal Detail:
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Title: Reproduction (Cambridge, England) Volume: - ISSN: 1741-7899 ISO Abbreviation: - Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-7-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100966036 Medline TA: Reproduction Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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N Ghanen, Animal Production Department, Faculty of Agriculture, Cairo University, Giza, Cairo, Egypt. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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