Document Detail

Bovine apolipoprotein B-100 is a dominant immunogen in therapeutic cell populations cultured in fetal calf serum in mice and humans.
MedLine Citation:
PMID:  17395779     Owner:  NLM     Status:  MEDLINE    
Recent studies have demonstrated that cell populations intended for therapeutic purposes that are cultured in heterologous animal products can acquire xenoantigens, potentially limiting their utility. In investigations of the immune response to murine embryonic stem cells, we found that a strong antibody response was generated after the second infusion. Both polyclonal and monoclonal antibody responses, derived from immunized mice, were found to be specific for bovine apolipoprotein B-100, which binds to abundant low-density lipoprotein receptors on the cell surface and is internalized. Here we show that in the majority of patients administered 3 different types of cell-based therapies using cells grown in fetal calf serum-containing media, an antibody response to bovine apolipoprotein B-100 develops after the second infusion and is the dominant specificity. The known and potential clinical effects of such antibodies are discussed.
Norihisa Sakamoto; Kazuhide Tsuji; Linda M Muul; Ann M Lawler; Emanuel F Petricoin; Fabio Candotti; Julia A Metcalf; Jorge A Tavel; H Clifford Lane; Walter J Urba; Bernard A Fox; Ajit Varki; Joan K Lunney; Amy S Rosenberg
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural     Date:  2007-03-29
Journal Detail:
Title:  Blood     Volume:  110     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-03     Completed Date:  2007-09-13     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  United States    
Other Details:
Languages:  eng     Pagination:  501-8     Citation Subset:  AIM; IM    
Division of Therapeutic Proteins, Center for Drug Evaluation and Research, U.S. Food and Drug Administration, Bethesda, MD 20892, USA.
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MeSH Terms
Antibody Formation
Antigens, Heterophile / immunology
Apolipoprotein B-100 / immunology*
Blood Banks
Embryonic Stem Cells / immunology
Fetal Blood / immunology*
Mice, Inbred C57BL
Mice, Inbred Strains / immunology
Mice, Transgenic
National Institutes of Health (U.S.)
United States
Reg. No./Substance:
0/Antigens, Heterophile; 0/Apolipoprotein B-100; 0/Vaccines

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