| Bovine Serum Albumin Oligomers in the E- and B-Forms at Low Protein Concentration and Ionic Strength. | |
| | |
MedLine Citation:
|
PMID: 23148944 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The manuscript describes the study of the oligomerization process of bovine serum albumin (BSA) in two different structural monomeric forms: the extended-form (E) at pH 2.0 and the basic-form (B) at pH 9.0. The study was conducted at low protein concentration (1mg/ml) and relatively short incubation time (maximum 56 days) in order to investigate early oligomerization events rather than the formation of mature fibrils. The comparison between the two isoforms show that oligomers form much faster (∼ 6 days) in the E-form than in the B-form where formation of oligomers requires ∼ 4 weeks. The oligomers appear to be limited to a maximum of tetramers with size<30nm. Hydrophobic interactions from exposed neutral amino acid residues in the elongated E-form are the likely cause for the quick formation of aggregates at acidic pH. We used an array of biophysical techniques for the study and determined that oligomerization occurs without further large changes in the secondary structure of the monomers. Under the conditions adopted in this study, aggregation does not seem to exceed the formation of tetramers, even though a very small amount of much larger aggregates seem to form. |
| | |
Authors:
|
Jeremiah Babcock; Lorenzo Brancaleon |
Related Documents
:
|
23554884 - Calcium induced regulation of skeletal troponin - computational insights from molecular... 23145064 - Structural basis for group b streptococcus pilus 1 sortases c regulation and specificity. 23000034 - Crystal structure of cytochrome p450 cyp105n1 from streptomyces coelicolor, an oxidase ... 23209794 - 1-anilino-8-naphthalene sulfonate (ans) is not a desirable probe for determining the mo... 15265034 - Mode of action of the microbial metabolite ge23077, a novel potent and selective inhibi... 15519164 - Pyrazole derivatives as new potent and selective 20-hydroxy-5,8,11,14-eicosatetraenoic ... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-11-10 |
Journal Detail:
|
Title: International journal of biological macromolecules Volume: - ISSN: 1879-0003 ISO Abbreviation: Int. J. Biol. Macromol. Publication Date: 2012 Nov |
Date Detail:
|
Created Date: 2012-11-14 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7909578 Medline TA: Int J Biol Macromol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012. Published by Elsevier B.V. |
Affiliation:
|
Department of Physics and Astronomy, University of Texas at San Antonio, One UTSA Circle, San Antonio, TX, 78242, United States. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Mechanisms, indications and results of salvage systemic therapy for sporadic and von Hippel-Lindau r...
Next Document: Sorption studies on heavy metal removal using chitin/bentonite biocomposite.