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Bovine Lactoferrin Can Be Taken Up By The Human Intestinal Lactoferrin Receptor And Exert Bioactivities.
MedLine Citation:
PMID:  21832946     Owner:  NLM     Status:  Publisher    
OBJECTIVES:: Human lactoferrin (hLF), a major protein in human milk, has been shown to exert multiple biological activities. To achieve some of the benefits of breast-fed infants, we therefore investigate the feasibility of adding commercially available bovine LF (CbLF) to infant formula. SUBJECTS AND METHODS:: An intestinal enterocyte model (Caco-2 cells) was used to compare the ability of bovine LF (bLF) purified by our lab and commercial bLF (CbLF) with hLF to resist digestion, bind to the receptor and exert bioactivities, including cellular proliferation, differentiation, IL-18 secretion and TGF-β1 expression. RESULTS:: bLf and CbLF, which are partially iron-saturated, can bind additional iron, partially resist digestion either dissolved in PBS or in the presence of infant formula at conditions similar to those of the infant gastrointestinal tract and bind to Caco-2 cells in a manner similar to hLF. bLF and CbLF as well as bound iron are also internalized by Caco-2 cells, as demonstrated by I-125 and Fe-59 labeling, albeit to somewhat less extent than hLF. CbLF promoted cell proliferation and differentiation to an extent similar to that of bLF and hLF, but these effects were not seen when the LF samples were saturated with iron (holo-LF). Native forms of hLF and bLF significantly increased expression of TGF-β1 and holo-forms of LFs stimulated IL-18 secretion significantly, with the highest results for CbLF. CONCLUSIONS:: CbLF is biologically active and is likely to exert several of the bioactivities of hLF if added to infant formula.
Bo Lönnerdal; Rulan Jiang; Xiaou Du
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-8-8
Journal Detail:
Title:  Journal of pediatric gastroenterology and nutrition     Volume:  -     ISSN:  1536-4801     ISO Abbreviation:  -     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-8-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8211545     Medline TA:  J Pediatr Gastroenterol Nutr     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Nutrition, University of California, Davis.
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