| Both transcriptional and posttranscriptional mechanisms regulate human telomerase template RNA levels. | |
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MedLine Citation:
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PMID: 10330139 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The human telomerase RNA component (hTR) is present in normal somatic cells at lower levels than in cancer-derived cell lines. To understand the mechanisms regulating hTR levels in different cell types, we have compared the steady-state hTR levels in three groups of cells: (i) normal telomerase-negative human diploid cells; (ii) normal cells transfected with the human telomerase catalytic subunit, hTERT; and (iii) cells immortalized in vitro and cancer cells expressing their own endogenous hTERT. To account for the differences in steady-state hTR levels observed in these cell types, we compared the transcription rate and half-life of hTR in a subset of these cells. The half-life of hTR in telomerase-negative cells is about 5 days and is increased 1.6-fold in the presence of hTERT. The transcription rate of hTR is essentially unchanged in cells expressing exogenous hTERT, and the increased steady-state hTR level appears to be due to the increased half-life. However, the transcription rate of hTR is greatly increased in cells expressing endogenous hTERT, suggesting some overlap in transcriptional regulatory control. We conclude that the higher hTR level in cells expressing an endogenous telomerase can be a result of both increased transcription and a longer half-life and that the longer half-life might be partially a result of protection or stabilization by the telomerase catalytic subunit. The 4-week half-life of hTR in H1299 tumor cells is the longest half-life yet reported for any RNA. |
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Authors:
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X Yi; V M Tesmer; I Savre-Train; J W Shay; W E Wright |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular and cellular biology Volume: 19 ISSN: 0270-7306 ISO Abbreviation: Mol. Cell. Biol. Publication Date: 1999 Jun |
Date Detail:
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Created Date: 1999-06-17 Completed Date: 1999-06-17 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 8109087 Medline TA: Mol Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3989-97 Citation Subset: IM |
Affiliation:
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Department of Cell Biology and Neuroscience, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235-9039, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Northern Cell Nucleus / metabolism Cell Transformation, Neoplastic Chromatography, Agarose DNA-Binding Proteins Epithelial Cells / metabolism Fibroblasts / metabolism Half-Life Humans Lung / metabolism Mice Models, Genetic RNA / physiology* RNA Processing, Post-Transcriptional / physiology* RNA, Messenger / metabolism RNA, Untranslated* Retroviridae / genetics Reverse Transcriptase Polymerase Chain Reaction Ribonucleases / metabolism Telomerase / genetics*, physiology* Templates, Genetic Time Factors Transcription, Genetic* Transfection Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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AG07992/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/RNA, Messenger; 0/RNA, Untranslated; 0/hTR RNA; 0/telomerase RNA; 63231-63-0/RNA; EC 2.7.7.49/Telomerase; EC 3.1.-/Ribonucleases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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