Document Detail

Both superficial and deep zone articular chondrocyte subpopulations exhibit the Crabtree effect but have different basal oxygen consumption rates.
MedLine Citation:
PMID:  20143333     Owner:  NLM     Status:  MEDLINE    
In the absence of in vivo measurements, the oxygen concentration within articular cartilage is calculated from the balance between cellular oxygen consumption and mass transfer. Current estimates of the oxygen tension within articular cartilage are based on oxygen consumption data from full-depth tissue samples. However, superficial and deep cell subpopulations of articular cartilage express intrinsic metabolic differences. We test the hypothesis that the subpopulations differ with respect to their intrinsic oxygen consumption rate. Chondrocytes from the full cartilage thickness demonstrate enhanced oxygen consumption when deprived of glucose, consistent with the Crabtree phenomena. Chondrocyte subpopulations differ in the prevailing availability of oxygen and glucose, which decrease with distance from the cartilage-synovial fluid interface. Thus, we tested the hypothesis that the oxygen consumption of each subpopulation is modulated by nutrient availability, by examining the expression of the Crabtree effect. The deep cells had a greater oxygen consumption than the superficial cells (V(max) of 6.6 compared to 3.2 fmol/cell/h), consistent with our observations of mitochondrial volume (mean values 52.0 vs. 36.4 microm(3)/cell). Both populations expressed the Crabtree phenomena, with oxygen consumption increasing approximately 2.5-fold in response to glycolytic inhibition by glucose deprivation or 2-deoxyglucose. Over 90% of this increase was oligomycin-sensitive and thus accounted for by oxidative phosphorylation. The data contributes towards our understanding of chondrocyte energy metabolism and provides information valuable for the accurate calculation of the oxygen concentration that the cells experience in vivo. The work has further application to the optimisation of bioreactor design and engineered tissues.
Hannah K Heywood; Martin M Knight; David A Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  223     ISSN:  1097-4652     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-04-07     Completed Date:  2010-04-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  630-9     Citation Subset:  IM    
Copyright Information:
(c) 2010 Wiley-Liss, Inc.
School of Engineering and Materials Science, Queen Mary University of London, London, UK.
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MeSH Terms
Adenosine Triphosphatases / metabolism
Cartilage, Articular / cytology*
Cell Hypoxia / drug effects
Chondrocytes / cytology*,  drug effects,  enzymology,  metabolism*
Glucose / deficiency,  pharmacology
Glycolysis / drug effects
Mitochondrial Size / drug effects
Models, Biological
Oxygen Consumption* / drug effects
Grant Support
080440/Z/06/Z//Wellcome Trust
Reg. No./Substance:
50-99-7/Glucose; EC 3.6.1.-/Adenosine Triphosphatases

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