Document Detail

Both ionically and enzymatically crosslinkable alginate-tyramine conjugate as materials for cell encapsulation.
MedLine Citation:
PMID:  17688281     Owner:  NLM     Status:  MEDLINE    
The swelling behavior of the structural material of cell-enclosing capsules is a key factor for the successful transplantation of these capsules in the treatment of diseases. The present study aimed to develop cell-enclosing capsules displaying minimal swelling under physiological conditions. We investigated the use of an alginate-tyramine conjugate synthesized by a carbodiimide/active-ester coupling reaction. The conjugate gel crosslinked by calcium ions and peroxidase-catalyzed oxidative coupling of phenols swelled less in saline than in unmodified alginate. The degree of swelling could be controlled by conjugate preparation conditions. The conjugate gel showed no obvious cytotoxicity for cells, including the process of oxidative coupling generation. Further, encapsulated cells could be cultured for up to 2 months and achieve approximately 5.2-fold greater mitochondrial activity after 51 days of encapsulation than after 1 day. These results show that this alginate-tyramine conjugate is a promising material for use in cell-enclosing capsules for cell therapy.
Shinji Sakai; Koei Kawakami
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  85     ISSN:  1552-4965     ISO Abbreviation:  -     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-02     Completed Date:  2008-06-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  345-51     Citation Subset:  IM    
Copyright Information:
Copyright 2007 Wiley Periodicals, Inc.
Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan.
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MeSH Terms
Alginates / chemical synthesis*,  chemistry
Carbodiimides / chemistry
Cell Line
Cells, Immobilized / cytology
Cross-Linking Reagents / chemistry
Glucuronic Acid / chemical synthesis,  chemistry
Hexuronic Acids / chemical synthesis,  chemistry
Materials Testing* / methods
Peroxidase / chemistry*
Tyramine / chemical synthesis*,  chemistry
Reg. No./Substance:
0/Alginates; 0/Carbodiimides; 0/Cross-Linking Reagents; 0/Gels; 0/Hexuronic Acids; 51-67-2/Tyramine; 576-37-4/Glucuronic Acid; 9005-32-7/alginic acid; EC

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