| Boswellia serrata and Salvia miltiorrhiza extracts reduce DMN-induced hepatic fibrosis in mice by TGF-beta1 downregulation. | |
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MedLine Citation:
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PMID: 23111960 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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BACKGROUND: </strong> Hepatic fibrosis is characterised by a progressive accumulation of fibrillar extracellular matrix (ECM) proteins, including collagen that occurs in chronic liver diseases. Transforming growth factor-beta1 (TGF-beta)/Smad3 signalling plays a major role in tissue fibrogenesis acting as a potent stimulus of ECM accumulation. <strong> AIM: </strong> To evaluate the effects of a combined therapy with anti-inflammatory Boswellia and anti-fibrotic Salvia extracts on the course of chronic hepatitis-associated fibrosis induced by dimethylnitrosamine (DMN) in mice, as well as on the hepatic expression of TGF-beta1 and Smad proteins. <strong> METHODS: </strong> Chronic hepatitis-associated fibrosis was induced in mice by intraperitoneal DMN administration. Mice were assigned to 5 groups: controls; DMN without any treatment; DMN treated orally with Boswellia extracts (50 mg/kg/day); DMN treated orally with Salvia extracts (150 mg/ kg/day); DMN treated orally with both Boswellia (50 mg/kg/day) and Salvia extracts (150 mg/kg/ day). The liver was excised for macroscopic examination and histological, morphometric and immunohistochemical (IHC) analyses. For IHC, alpha-smooth muscle actin (alpha-SMA), collagen types I-III, TGF-beta1, connective tissue growth factor (CTGF), Smad3, Smad7, CD3, PCNA and TUNEL antibodies were used. <strong> RESULTS: </strong> The combined oral administration of Boswellia and Salvia extracts improved the course and macroscopic findings of DMN-induced chronic hepatitis-associated fibrosis. The histological severity of the hepatic fibrosis showed a marked improvement following treatment and was associated with a reduction in the hepatic expression of alpha-SMA, collagen I-III, CTGF, TGF-beta1, Smad3, and Smad7. <strong> CONCLUSIONS: </strong> These data demonstrate that co-treatment of Boswellia plus Salvia extracts is effective in preventing hepatic fibrosis in DMN-induced chronic hepatitis. The anti-fibrotic properties are mainly related to Salvia extracts and appear to be mediated by the inhibition of the TGF-beta1/Smad3 pathway. |
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Authors:
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R Sferra; A Vetuschi; V Catitti; S Ammanniti; S Pompili; D Melideo; G Frieri; E Gaudio; G Latella |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: European review for medical and pharmacological sciences Volume: 16 ISSN: 1128-3602 ISO Abbreviation: Eur Rev Med Pharmacol Sci Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-10-31 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9717360 Medline TA: Eur Rev Med Pharmacol Sci Country: Italy |
Other Details:
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Languages: eng Pagination: 1484-98 Citation Subset: IM |
Affiliation:
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Department of Experimental Medicine, University of L'Aquila, L'Aquila, Italy. giolatel@tin.it Department of Internal Medicine, GI Unit, University of L'Aquila, L'Aquila, Italy Department of Human Anatomy, Sapienza University of Rome, Rome, Italy. |
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