Document Detail

Borrelia burgdorferi stimulates in vitro a selective expansion of CD3-CD4-CD8- (triple negative) autoreactive cells in naive rhesus monkey lymphocytes.
MedLine Citation:
PMID:  11262204     Owner:  NLM     Status:  MEDLINE    
Autoreactive cell lines were generated from cell suspensions of freshly isolated naive monkey lymph node (LN) cells and peripheral blood mononuclear cells by cocultivation with freeze-thawed Borrelia burgdorferi spirochetes (Bb/FT). These cells produced interleukin (IL)-6 when cocultured with autologous antigen-presenting cells (APCs) alone without Bb/FT. IL-6 production was not observed when control cell lines were stimulated in the same fashion. CD4+-enriched T cell populations obtained from the LN autoreactive cell line also produced IL-6 when cultivated with APCs alone. When these cells were cultivated further in the presence of APCs, a population of cells whose phenotype was CD56+/-CD4-CD8-CD3- was predominantly selected. These cells both proliferated and produced IL-6 when cocultured with APCs alone. The possible relevance of these cells to Lyme disease pathogenesis remains to be determined.
F Ganapamo; V A Dennis; M T Philipp
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.     Date:  2001-03-16
Journal Detail:
Title:  The Journal of infectious diseases     Volume:  183     ISSN:  0022-1899     ISO Abbreviation:  J. Infect. Dis.     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-03-23     Completed Date:  2001-05-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0413675     Medline TA:  J Infect Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1221-8     Citation Subset:  AIM; IM    
Department of Parasitology, Tulane Regional Primate Research Center, Tulane University Health Sciences Center, 18703 Three Rivers Road, Covington, LA 70433, USA.
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MeSH Terms
Antigen-Presenting Cells / cytology,  immunology
Antigens, CD3 / analysis
Antigens, CD4 / analysis
Antigens, CD8 / immunology
Borrelia burgdorferi Group / immunology*
CD4-Positive T-Lymphocytes / immunology
Cells, Cultured
Coculture Techniques
Concanavalin A
Flow Cytometry
Interleukin-2 / pharmacology
Interleukin-6 / biosynthesis
Lymph Nodes / immunology*
Lymphocyte Activation / drug effects
Macaca mulatta
T-Lymphocytes / cytology,  immunology,  microbiology*
Grant Support
Reg. No./Substance:
0/Antigens, CD3; 0/Antigens, CD4; 0/Antigens, CD8; 0/Interleukin-2; 0/Interleukin-6; 11028-71-0/Concanavalin A

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