| Borrelia burgdorferi bind to epithelial cell proteoglycans. | |
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MedLine Citation:
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PMID: 8113413 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Borrelia burgdorferi adhere to mammalian cells in vitro but neither the ligand(s) nor the receptor(s) has (have) been clearly established. Using an in vitro attachment-inhibition assay, a B. burgdorferi attachment mechanism has been identified. Heparin, heparan sulfate, and dermatan sulfate reduced the attachment of virulent B. burgdorferi strain 297 to HeLa cells by approximately 60%. In addition, virulent, but not avirulent, B. burgdorferi strains B31, N40, and HB19 demonstrated heparin attachment-inhibition. Attachment to Chinese hamster ovary cells deficient in heparan sulfate proteoglycans was reduced by 68% compared to attachment to wild-type cells and was identical to attachment at maximum heparin inhibition to the wild-type cells. Pretreatment of HeLa cell monolayers with heparitinase, heparinase, and chondroitinase ABC, but not with chondroitinase AC, reduced borrelial attachment by approximately 50%. A moderately high affinity, low copy number, promiscuous B. burgdorferi glycosaminoglycan receptor was demonstrated by equilibrium binding studies. A 39-kD polypeptide, purified by heparin affinity chromatography from Triton X-100 extracts derived from virulent borrelia, was a candidate for this receptor. These studies indicate that one mode of B. burgdorferi attachment to eukaryotic cells is mediated by a borrelial glycosaminoglycan receptor attaching to surface-exposed proteoglycans on mammalian cells. |
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Authors:
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R D Isaacs |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of clinical investigation Volume: 93 ISSN: 0021-9738 ISO Abbreviation: J. Clin. Invest. Publication Date: 1994 Feb |
Date Detail:
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Created Date: 1994-03-30 Completed Date: 1994-03-30 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7802877 Medline TA: J Clin Invest Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 809-19 Citation Subset: AIM; IM |
Affiliation:
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Department of Medicine, Department of Veterans Affairs Medical Center, Jackson, Mississippi 39216. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacterial Adhesion* Bacterial Proteins / isolation & purification, metabolism Borrelia burgdorferi Group / drug effects, pathogenicity, physiology* CHO Cells Cell Line Cricetinae Electrophoresis, Polyacrylamide Gel Epithelium / microbiology, physiology Glycosaminoglycans / pharmacology* Hela Cells Heparin / metabolism Humans Kinetics Mammals Molecular Weight Proteoglycans / metabolism* Receptors, Cell Surface / isolation & purification, metabolism* Virulence |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Glycosaminoglycans; 0/Proteoglycans; 0/Receptors, Cell Surface; 0/glycosaminoglycan receptor; 9005-49-6/Heparin |
| Comments/Corrections | |
Comment In:
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J Clin Invest. 1994 Feb;93(2):460
[PMID:
8113383
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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