Document Detail


Boronic acid-lectin affinity chromatography. 1. Simultaneous glycoprotein binding with selective or combined elution.
MedLine Citation:
PMID:  17909756     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We introduce a novel combination of boronic acid affinity chromatography with lectin affinity chromatography, dubbed as boronic acid-lectin affinity chromatography (BLAC). Concanavalin A and wheat germ agglutinin lectins were mixed with the pesudo-lectin boronic acid to form the BLAC affinity column and their performance was evaluated with standard glycoproteins. Optimization of the binding and elution buffers for the BLAC system is described. The BLAC columns were employed to isolate glycoproteins of interest using both selective and/or combined elution.
Authors:
Alex Monzo; Guenther K Bonn; András Guttman
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-02
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  389     ISSN:  1618-2650     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-11-30     Completed Date:  2008-02-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2097-102     Citation Subset:  IM    
Affiliation:
Horváth Laboratory of Bioseparation Sciences, Institute of Analytical Chemistry and Radiochemistry, University of Innsbruck, Innrain 66, 6020, Innsbruck, Austria.
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MeSH Terms
Descriptor/Qualifier:
Boronic Acids / chemistry*
Buffers
Chromatography, Affinity / methods*
Concanavalin A / chemistry
Glycoproteins / analysis,  chemistry*,  metabolism
Lectins / chemistry*
Protein Binding
Wheat Germ Agglutinins / chemistry
Chemical
Reg. No./Substance:
0/Boronic Acids; 0/Buffers; 0/Glycoproteins; 0/Lectins; 0/Wheat Germ Agglutinins; 11028-71-0/Concanavalin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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