Document Detail


Boromycin abrogates bleomycin-induced G2 checkpoint.
MedLine Citation:
PMID:  15638327     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The DNA-damaging agent bleomycin arrests the cell cycle at the G2 phase of Jurkat cells defective in the G1 checkpoint, and microtubule-acting colchicine arrests it at the M phase. Boromycin itself, an actinomycete metabolite, showed no effect on the cell cycle status of Jurkat cells at least up to 340 nM. However, the compound (3.4-340 nM) was found to abrogate bleomycin-induced G2 arrest even at 3.4 nM, resulting in a drastic decrease in cells at the G2 phase and increase in cells at the subG1 phase. On the other hand, boromycin did not show any effect on the colchicine-induced M phase arrest in Jurkat cells, nor on the cell cycle status of the bleomycin-treated or -untreated HUVEC, normal cells conserving both G1 and G2 checkpoints. Furthermore, boromycin potentiated anti-tumor activity of bleomycin in scid mice inoculated with Jurkat cells. These data suggest that boromycin disrupts the cell cycle at the G2 checkpoint of cancer cells selectively, leading to sensitization of cancer cells to anti-cancer reagents.
Authors:
Masayoshi Arai; Yukio Koizumi; Hitoshi Sato; Takumi Kawabe; Masashi Suganuma; Hidetaka Kobayashi; Hiroshi Tomoda; Satoshi Omura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of antibiotics     Volume:  57     ISSN:  0021-8820     ISO Abbreviation:  J. Antibiot.     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2005-01-10     Completed Date:  2005-02-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0151115     Medline TA:  J Antibiot (Tokyo)     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  662-8     Citation Subset:  IM    
Affiliation:
Kitasato Institute for Life Sciences & Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane Minato-ku, Tokyo 108-8641, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bleomycin / pharmacology*
Borates / pharmacology*
G2 Phase / drug effects*
Humans
Jurkat Cells
Male
Mice
Mice, SCID
Chemical
Reg. No./Substance:
0/Borates; 11056-06-7/Bleomycin; 34524-20-4/boromycin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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