Document Detail

Bordetella pertussis adenylate cyclase. Penetration into host cells.
MedLine Citation:
PMID:  2900763     Owner:  NLM     Status:  MEDLINE    
Exposure of Chinese hamster ovary, mouse adrenal cortex tumor (Y-1), THP-1 and U-937 cells and human erythrocytes to adenylate-cyclase-containing urea extracts of Bordetella pertussis (strain 114) organisms promotes the formation of large concentrations of intracellular cAMP. Accumulation is dependent on dose and temperature, with significant accumulation occurring at 4 degrees C, and is virtually instantaneous, with a doubling at 1 min. There is an absolute Ca2+ requirement but external calmodulin (the activator of cyclase activity) has no effect except in erythrocytes and U-937 cells, where it reduces cAMP accumulation. However, calmodulin antagonists inhibit cAMP accumulation. In Y-1 adrenal cells the urea-extract adenylate cyclase stimulates steroidogenesis. Anti-(B. pertussis) antibodies inhibit cyclase activity and prevent further cAMP accumulation after 10 min in cells previously exposed to urea extract. The same effect is obtained by washing. This suggests that a portion of the cyclase is associated with cells in a form not accessible to antibody or washing but accessible to substrate, which we interpret as internalized enzyme with a short lifetime. Continuing cAMP accumulation thus appears to need a continuing source of external cyclase. Inhibitors of the effect of diphtheria toxin, such as NH4Cl, methylamine, chloroquine or monensin, have no inhibitory effect on the accumulation of intracellular cAMP promoted by the internalized adenylate cyclase of urea extracts of B. pertussis organisms. We conclude that entry of the cyclase into cells is not by receptor-mediated endocytosis.
F Gentile; A Raptis; L G Knipling; J Wolff
Related Documents :
8023893 - Principal cell-specific antigen and hormonal regulatory network in rc.svtsa58 cell line.
20453 - Sonication as a tool for the study of adenylyl cyclase activity.
1520303 - Tgf beta inhibits rat thyroid cell proliferation without alterations in the expression ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  175     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1988 Aug 
Date Detail:
Created Date:  1988-10-11     Completed Date:  1988-10-11     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  447-53     Citation Subset:  IM    
National Institute of Diabetes, Digestive and Kidney Diseases, Bethesda, Maryland 20892.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adenylate Cyclase / analysis,  metabolism*
Adenylate Cyclase Toxin
Adrenal Cortex / metabolism
Antibodies, Bacterial / pharmacology
Bordetella pertussis / enzymology*,  immunology
Calmodulin / pharmacology
Cell Line
Cell Membrane Permeability
Cyclic AMP / biosynthesis
Endocytosis / drug effects
Erythrocytes / metabolism
Ovary / metabolism
Virulence Factors, Bordetella / pharmacology
Reg. No./Substance:
0/Adenylate Cyclase Toxin; 0/Antibodies, Bacterial; 0/Calmodulin; 0/Virulence Factors, Bordetella; 60-92-4/Cyclic AMP; EC Cyclase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Molecular analysis of formaldehyde-induced mutations in human lymphoblasts and E. coli.
Next Document:  Effect of intracellular pH and potassium ions on a primary transport system for glutamate/aspartate ...