Document Detail

Bone-specific overexpression of NPY modulates osteogenesis.
MedLine Citation:
PMID:  23196263     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: Neuropeptide Y (NPY) is a peptide involved in the regulation of appetite and energy homeostasis. Genetic data indicates that NPY decreases bone formation via central and peripheral activities. NPY is produced by various cell types including osteocytes and osteoblasts and there is evidence suggesting that peripheral NPY is important for regulation of bone formation. We sought to investigate the role of bone-derived NPY in bone metabolism.
METHODS: We generated a mouse where NPY was over-expressed specifically in mature osteoblasts and osteocytes (Col2.3NPY) and characterized the bone phenotype of these mice in vivo and in vitro.
RESULTS: Trabecular and cortical bone volume was reduced in 3-month-old animals, however bone formation rate and osteoclast activity were not significantly changed. Calvarial osteoblast cultures from Col2.3NPY mice also showed reduced mineralization and expression of osteogenic marker genes.
CONCLUSIONS: Our data suggest that osteoblast/osteocyte-derived NPY is capable of altering osteogenesis in vivo and in vitro and may represent an important source of NPY for regulation of bone formation. However, it is possible that other peripheral sources of NPY such as the sympathetic nervous system and vasculature also contribute to peripheral regulation of bone turnover.
I Matic; B G Matthews; T Kizivat; J C Igwe; I Marijanovic; S T Ruohonen; E Savontaus; D J Adams; I Kalajzic
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of musculoskeletal & neuronal interactions     Volume:  12     ISSN:  1108-7161     ISO Abbreviation:  J Musculoskelet Neuronal Interact     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-30     Completed Date:  2013-05-17     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101084496     Medline TA:  J Musculoskelet Neuronal Interact     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  209-18     Citation Subset:  IM    
Department of Reconstructive Sciences, University of Connecticut Health Center, Farmington, CT 06032, USA.
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MeSH Terms
Bone and Bones / metabolism*
Mice, Transgenic
Neuropeptide Y / genetics*,  metabolism
Osteoblasts / metabolism*
Osteocytes / metabolism*
Osteogenesis / physiology*
Grant Support
Reg. No./Substance:
0/Neuropeptide Y

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