Document Detail

Bone sialoprotein-induced reparative dentinogenesis in the pulp of rat's molar.
MedLine Citation:
PMID:  11218498     Owner:  NLM     Status:  MEDLINE    
Bone sialoprotein (BSP), an osteogenic protein (OP), mixed with a carrier, was implanted in the pulp of rat first upper molars (OP group). Cavities were prepared with dental burs and pulp perforation was carried out by pressure with the tip of a steel probe. After 8, 14, and 30 days, the rats were killed and the pulps of the OP group were compared with (1) a sham group (S group), (2) a group where the carrier was implanted alone (C group), and (3) capping with calcium hydroxide (Ca group). After 8 days, a few inflammatory cells were seen, mostly located at the pulp surface near the perforation. In the Ca group, a dentin bridge started to form, in contrast to the other groups. After 15 days, globular structures were seen in the pulps of the S and C groups. A reparative osteodentin bridge isolated the pulp from the cavity in the Ca group. Variable reactions were seen in the OP group, with some evidence of cell and matrix alignments or plugs of osteodentin in continuity with an inner layer of reparative dentin. After 30 days, irregular osteodentin formation was observed in the pulps of the S and C groups, with a tendency for globular structures to merge, but with interglobular spaces filled by pulp remnants. In the Ca group, osteodentin was observed in the mesial part of the pulp chamber. In the BSP-implanted group, the osteogenic protein stimulated the formation of a homogeneous dentin-like deposit occupying most of the mesial part of the pulp. Apparently, BSP stimulates the differentiation of cells which secrete an organized extracellular matrix more efficiently than any other capping material used so far. Altogether, the results reported here support that bone sialoprotein displays novel bioactive properties and is capable of stimulating in 1 month's time the development of a thick reparative dentinal tissue in the pulp, occluding the perforation and filling the mesial third of the pulp chamber.
F Decup; N Six; B Palmier; D Buch; J J Lasfargues; E Salih; M Goldberg
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Clinical oral investigations     Volume:  4     ISSN:  1432-6981     ISO Abbreviation:  Clin Oral Investig     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2001-02-20     Completed Date:  2001-03-15     Revised Date:  2010-02-04    
Medline Journal Info:
Nlm Unique ID:  9707115     Medline TA:  Clin Oral Investig     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  110-9     Citation Subset:  D    
Laboratoire de Biologie et Physiopathologie Cranio-Faciale, Faculté de Chirurgie Dentaire-Paris V, 1 rue Maurice Arnoux, 92120 Montrouge, France.
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MeSH Terms
Calcification, Physiologic
Calcium Hydroxide / therapeutic use
Cell Differentiation / drug effects
Collagen / therapeutic use
Dental Pulp / drug effects*
Dental Pulp Capping
Dental Pulp Cavity / drug effects
Dental Pulp Exposure / therapy
Dentin, Secondary / chemically induced
Dentinogenesis / drug effects*
Disease Models, Animal
Drug Carriers
Extracellular Matrix / drug effects
Follow-Up Studies
Glass Ionomer Cements
Odontoblasts / drug effects
Rats, Sprague-Dawley
Root Canal Filling Materials
Sialoglycoproteins / therapeutic use*
Reg. No./Substance:
0/Drug Carriers; 0/Glass Ionomer Cements; 0/Root Canal Filling Materials; 0/Sialoglycoproteins; 0/integrin-binding sialoprotein; 1305-62-0/Calcium Hydroxide; 9000-70-8/Gelatin; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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