Document Detail


Bone regeneration in distraction osteogenesis demonstrates significantly increased vascularity in comparison to fracture repair in the mandible.
MedLine Citation:
PMID:  22337436     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Tissue analysis of bone regenerate has suggested an intense vascular response after mandibular distraction osteogenesis (DO). Quantifying and three-dimensionally imaging this vascular response could be of immense clinical import in efforts to advance the utility of bone regeneration and repair. Conventional quantification of vascular responses has heretofore focused on inexact, cumbersome measurements of blood flow and histologic vessel counting. Using micro-computed tomography after vessel perfusion, we posit that quantitative vascular metrics will be significantly higher in mandibular DO compared with those observed in fracture repair (FxR) after bony union.
METHODS: Sprague-Dawley rats underwent mandibular osteotomy and external fixator placement. A DO group (n=9) underwent a 5.1-mm distraction, whereas a FxR group (n=12) had a 2.1-mm fixed gap set. Forty days after surgery, Microfil was perfused into the vasculature, and imaging ensued. Vascular radiomorphometrics were calculated for the regions of interest. Independent-samples t-test was performed for comparison, with statistical significance set at P≤0.05.
RESULTS: Stereological analysis demonstrated statistically significant increases in the distracted vasculature compared with fracture repair: vessel volume fraction (5.4% versus 2.8%, P=0.030) and vessel number (0.86 versus 0.50 mm, P=0.014).
CONCLUSIONS: We report robust and quantifiable increases in vascular density in DO compared with FxR. Our findings support a significant distinction between the regenerative processes of mandibular DO from the reparative mechanisms controlling fracture healing. A better understanding of the differences between the 2 types of bone formation may enable clinicians to selectively optimize therapeutic outcomes in the future.
Authors:
Alexis Donneys; Catherine N Tchanque-Fossuo; Aaron S Farberg; Sagar S Deshpande; Steven R Buchman
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  The Journal of craniofacial surgery     Volume:  23     ISSN:  1536-3732     ISO Abbreviation:  J Craniofac Surg     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-02-16     Completed Date:  2012-07-24     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  9010410     Medline TA:  J Craniofac Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  328-32     Citation Subset:  D    
Affiliation:
Craniofacial Research Laboratory, Plastic Surgery Section, University of Michigan Ann Arbor, Michigan 28109-4217, USA.
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MeSH Terms
Descriptor/Qualifier:
Angiography / methods
Animals
Bone Regeneration / physiology*
External Fixators
Fixatives / chemistry
Formaldehyde / chemistry
Fracture Healing / physiology*
Imaging, Three-Dimensional / methods
Male
Mandible / blood supply*
Mandibular Fractures / surgery*
Microvessels / radiography
Osteogenesis, Distraction / instrumentation,  methods*
Osteotomy / methods
Rats
Rats, Sprague-Dawley
Silicone Elastomers / chemistry
Time Factors
X-Ray Microtomography / methods
Grant Support
ID/Acronym/Agency:
R01 CA 12587-01/CA/NCI NIH HHS; R01 CA125187/CA/NCI NIH HHS; T32 GM008616/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Fixatives; 0/Silicone Elastomers; 50-00-0/Formaldehyde
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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