| Bone morphogenetic proteins mediate cellular response and, together with Noggin, regulate astrocyte differentiation after spinal cord injury. | |
| | |
MedLine Citation:
|
PMID: 20005873 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Bone morphogenetic proteins (BMPs) play a critical role in regulating cell fate determination during central nervous system (CNS) development. In light of recent findings that BMP-2/4/7 expressions are upregulated after spinal cord injury, we hypothesized that the BMP signaling pathway is important in regulating cellular composition in the injured spinal cord. We found that BMP expressions were upregulated in neural stem cells (NSCs), neurons, oligodendrocytes and microglia/macrophages. Increased expression levels of pSmad1/5/8 (downstream molecules of BMP) were detected in neurons, NSCs, astrocytes, oligodendrocytes and oligodendroglial progenitor cells (OPCs). Active astrocytes which form the astroglial scar were probably derived from NSCs, OPCs and resident astrocytes. Since quiescent NSCs in the normal adult spinal cord will proliferate and differentiate actively into neural cells after traumatic injury, we proposed that BMPs can regulate cellular components by controlling NSC differentiation. Neurosphere culture from adult mouse spinal cord showed that BMP-4 promoted astrocyte differentiation from NSCs while suppressing production of neurons and oligodendrocytes. Conversely, inhibition of BMP-4 by Noggin notably decreased the ratio of astrocyte to neuron numbers. However, intrathecal administration of Noggin in the injured spinal cord failed to attenuate glial fibrillar acidic protein (GFAP) expression even though it effectively reduced pSmad expression. Noggin treatment did not block phosphorylation of Stat3 and the induction of GFAP in the injured spinal cord, suggesting that in addition to the BMP/Smad pathway, the JAK/STAT pathway may also be involved in the regulation of GFAP expression after spinal cord injury. |
| | |
Authors:
|
Qi Xiao; Yang Du; Wutian Wu; Henry K Yip |
Related Documents
:
|
8793863 - Developmental and fgf-2-mediated regulation of syndecans (1-4) and glypican in oligoden... 15782413 - Spatial and temporal expression of s100b in cells of oligodendrocyte lineage. 17481363 - Analysis and quantitation of nf-kappab nuclear translocation in tumor necrosis factor a... |
Publication Detail:
|
Type: Journal Article Date: 2009-12-11 |
Journal Detail:
|
Title: Experimental neurology Volume: 221 ISSN: 1090-2430 ISO Abbreviation: Exp. Neurol. Publication Date: 2010 Feb |
Date Detail:
|
Created Date: 2010-01-27 Completed Date: 2010-02-16 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0370712 Medline TA: Exp Neurol Country: United States |
Other Details:
|
Languages: eng Pagination: 353-66 Citation Subset: IM |
Copyright Information:
|
Copyright 2009 Elsevier Inc. All rights reserved. |
Affiliation:
|
Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Astrocytes / drug effects* Bone Morphogenetic Proteins / pharmacology*, therapeutic use* Bromodeoxyuridine / metabolism Carrier Proteins / pharmacology*, therapeutic use* Cell Differentiation / drug effects* Cell Proliferation / drug effects Cells, Cultured Disease Models, Animal Female Gene Expression Regulation / drug effects Mice Mice, Inbred C57BL Myelin Basic Proteins / genetics, metabolism Nerve Tissue Proteins / genetics, metabolism Neurons Nuclear Proteins / genetics, metabolism Oligodendroglia / drug effects RNA, Messenger / metabolism Signal Transduction / drug effects, physiology Spinal Cord Injuries* / drug therapy, pathology, physiopathology Stem Cells / drug effects Time Factors |
| Chemical | |
Reg. No./Substance:
|
0/Bone Morphogenetic Proteins; 0/Carrier Proteins; 0/Myelin Basic Proteins; 0/Nerve Tissue Proteins; 0/Nuclear Proteins; 0/RNA, Messenger; 148294-77-3/noggin protein; 59-14-3/Bromodeoxyuridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Mesoderm and ectoderm lineages in the crustacean Parhyale hawaiensis display intra-germ layer compen...
Next Document: Application of small interfering RNAs modified by unlocked nucleic acid (UNA) to inhibit the heart-p...