Document Detail

Bone morphogenetic protein signaling is essential for terminal differentiation of the intestinal secretory cell lineage.
MedLine Citation:
PMID:  17678919     Owner:  NLM     Status:  MEDLINE    
BACKGROUND & AIMS: Bone morphogenetic proteins (Bmps) are morphogens known to play key roles in gastrointestinal development and pathology. Most Bmps are produced primarily by the mesenchymal compartment and activate their signaling pathways following a paracrine or autocrine route. The aim of this study was to investigate the role of epithelial Bmp signaling in intestinal morphogenesis and maintenance of adult epithelial cell functions.
METHODS: With the use of tissue-specific gene ablation, we generated mice lacking the Bmp receptor type IA (Bmpr1a) exclusively in the intestinal epithelium. Bmpr1a mutant and control mice were sacrificed for histology, immunofluorescence, Western blot analysis, electron microscopy, and quantitative polymerase chain reaction.
RESULTS: As well as showing increased proliferation and altered intestinal epithelial morphology, Bmpr1a mutant mice revealed that epithelial Bmp signaling is associated with impaired terminal differentiation of cells from the secretory lineage but not with the determination of cell fate. Loss of Bmp signaling exclusively in the epithelial compartment is not sufficient for the initiation of the de novo crypt phenomenon associated with juvenile polyposis syndrome.
CONCLUSIONS: Epithelial Bmp signaling plays an important role in the terminal differentiation of the intestinal secretory cell lineage but not in de novo crypt formation. These findings emphasize the importance of delineating the contribution of the stroma vs the epithelium in gastrointestinal physiology and pathology.
Benoit A Auclair; Yannick D Benoit; Nathalie Rivard; Yuji Mishina; Nathalie Perreault
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't     Date:  2007-07-03
Journal Detail:
Title:  Gastroenterology     Volume:  133     ISSN:  0016-5085     ISO Abbreviation:  Gastroenterology     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-09-14     Completed Date:  2007-10-23     Revised Date:  2012-06-25    
Medline Journal Info:
Nlm Unique ID:  0374630     Medline TA:  Gastroenterology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  887-96     Citation Subset:  AIM; IM    
Canadian Institutes of Health Research Team on Digestive Epithelium, Département d'Anatomie et Biologie Cellulaire, Faculté de Médecine et des Sciences de la Santé, Université de Sherbrooke, Sherbrooke, Quebec, Canada.
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MeSH Terms
Bone Morphogenetic Protein Receptors, Type I / genetics,  metabolism
Bone Morphogenetic Proteins / genetics,  metabolism*
Cell Differentiation / physiology*
Cell Proliferation
Intestinal Mucosa / cytology,  metabolism
Jejunum / cytology*,  metabolism*,  pathology
Mice, Inbred Strains
Mice, Transgenic
Mutation / genetics
Signal Transduction / physiology*
Wnt Proteins / physiology
beta Catenin / physiology
Reg. No./Substance:
0/Bone Morphogenetic Proteins; 0/Wnt Proteins; 0/beta Catenin; EC protein, mouse; EC Morphogenetic Protein Receptors, Type I
Comment In:
Gastroenterology. 2007 Sep;133(3):1035-8   [PMID:  17854606 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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